IL-17F Polyclonal antibody

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  WB analysis using 30589-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30589-1-AP (IL-17F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IL-17F Polyclonal Antibody for WB, ELISA

Cat No. 30589-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Il17f, interleukin 17F, IL 17F, C87042

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, EL-4 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30589-1-AP targets IL-17F in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 17F
• Calculated Molecular Weight: 17KD
• Observed Molecular Weight: 17-25 kDa
• GenBank Accession Number: NM_145856.2
• Gene Symbol: IL-17F
• Gene ID (NCBI): 257630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7TNI7-2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-17F, which is expressed by helper T cell 17(Th17) (at protein level). The expression pattern reflects the differentiation state. In fully differentiated Th17 cells, the level of IL-17a-IL-17f heterodimers is higher than that of IL-17a-IL-17a and IL-17f-IL-17f dimers (PMID:18025225), which are mainly secreted in the intestine (PMID:29915298). It is expressed by resident cells, epithelial cells and immune cell subsets of lamina propria (including natural killer cells, dendritic cells, macrophages and various T and B cell subsets) (PMID:16990136, PMID:29915298). The molecular weight of IL-17F is 17 kDa. IL-17F has the strongest similarity with IL-17A, and forms homodimer or heterodimer with IL-17A.