IL-17F Recombinant monoclonal antibody, PBS Only

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  WB analysis using 87510-1-RR (same clone as 87510-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87510-1-RR (IL17F antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87510-1-PBS in a different storage buffer formulation.

• 

  Affinity of 87510-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87510-1-RR against Human IL-17F were performed. The affinity constant is 43.1 nM.

IL-17F Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87510-1-PBS

Clone No.252565B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

IL17F, Cytokine ML 1, Cytokine ML-1, IL 17F, interleukin 17F

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Product Information

87510-1-PBS targets IL-17F in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2823

  Product name: recombinant human IL17F protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 31-163 aa of NM_052872.3

  Sequence: RKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHVQ

• Full Name: interleukin 17F
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: NM_052872.3
• Gene Symbol: IL-17F
• Gene ID (NCBI): 112744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96PD4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The interleukin 17 (IL-17) family of cytokines contains 6 structurally related cytokines, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 family plays crucial roles in host defense against microbial organisms and in the development of inflammatory diseases. IL-17A is a pro-inflammatory cytokine that also has the capacity to promote angiogenesis and osteoclastogenesis. IL-17F shares the highest homology with IL-17A and signals via a receptor composed by the IL-17RA and IL-17RC subunits. IL-17A and IL-17F can form IL-17A/A or IL-17F/F homodimers, IL-17A/F heterodimers are also formed. IL-17A and IL-17F, produced by the Th17 CD4(+) T cell lineage, have been linked to a variety of inflammatory and autoimmune conditions. IL-17F levels are elevated in sera and lesional psoriatic skin compared to non-lesional tissue. IL-17F also has been implicated in the development of neutrophilic airway inflammation.