Anti-Human IL-5 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of human PBMCs using 98178-1-RR (same clone as 98178-1-PBS)

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-5 Rabbit Recombinant Antibody (98178-1-RR, Clone: 240915D12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98178-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98178-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98178-1-RR against Human IL-5 were performed. The affinity constant is 0.689 nM.

IL-5 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98178-1-PBS

Clone No.240915D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

IL5, 240915D12, B-cell differentiation factor I, IL 5, Interleukin 5

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Product Information

98178-1-PBS targets IL-5 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0380

  Product name: Recombinant Human IL-5 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 20-134 aa of BC066282

  Sequence: IPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES

• Full Name: interleukin 5 (colony-stimulating factor, eosinophil)
• Calculated Molecular Weight: 134 aa, 15 kDa
• GenBank Accession Number: BC066282
• Gene Symbol: IL5
• Gene ID (NCBI): 3567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P05113
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IL-5 is a 4-α-helix, pleiotropic cytokine that regulates the activities of B cells, eosinophils, and basophils. It is produced mostly by CD4+ T cells, eosinophils, and basophils, and signals through its specific receptor (IL-5R) on target cells to activate downstream JAK/STAT, Btk, and Ras/ERk pathways. Due to its pleiotropic effects, IL-5 plays a role in both innate and adaptive immune responses. It is a strong promoter of eosinophil differentiation, migration, maturation, and survival. Additionally, IL-5 is also capable of stimulating activated B cells to differentiate into antibody-secreting cells. Elevated IL-5 levels are strongly correlated with eosinophilia, which is a hallmark feature of allergy, asthma, and hypersensitivity. (PMID: 21986312, 22174445, 19819937, 16895557)