IL-5RA Polyclonal antibody, PBS Only

• 

  WB analysis of human peripheral blood platelets using 12655-1-AP (same clone as 12655-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12655-1-AP (IL-5RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12655-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 12655-1-AP (same clone as 12655-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12655-1-AP (IL5RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12655-1-PBS in a different storage buffer formulation.

IL-5RA Polyclonal Antibody for WB, Indirect ELISA

Cat No. 12655-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

IL5RA, IL 5RA, IL 5R subunit alpha, IL 5R alpha, IL 5 receptor subunit alpha

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Product Information

12655-1-PBS targets IL-5RA in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-5RA fusion protein Ag3358
• Full Name: interleukin 5 receptor, alpha
• Calculated Molecular Weight: 48 kDa, 38 kDa
• Observed Molecular Weight: 70-75 kDa, 43 kDa
• GenBank Accession Number: BC027599
• Gene Symbol: IL-5RA
• Gene ID (NCBI): 3568
• RRID: AB_2127342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01344
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-5 receptor subunit alpha (IL-5RA), also known as CD125, is a type I transmembrane protein that belongs to the type I cytokine receptor family. IL-5RA is a critical component of the high-affinity IL-5 receptor complex, IL-5R, which consists of the alpha chain (IL-5RA) that specifically binds to IL-5 and the common beta chain (CD131) shared with receptors for IL-3 and GM-CSF (PMID: 1833065; 7872157). IL-5/IL-5RA signaling axis is crucial for the differentiation, proliferation, recruitment, and activation of eosinophils, and is also involved in pathological eosinophilia (PMID: 34638583).