IL-5RA Polyclonal antibody

IL-5RA Polyclonal Antibody for WB, ELISA
Cat No. 12655-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

IL5RA, IL 5RA, IL 5R subunit alpha, IL 5R alpha, IL 5 receptor subunit alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inhuman peripheral blood platelets, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12655-1-AP targets IL-5RA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-5RA fusion protein Ag3358 Predict reactive species
Full Name interleukin 5 receptor, alpha
Calculated Molecular Weight 48 kDa, 38 kDa
Observed Molecular Weight 70-75 kDa, 43 kDa
GenBank Accession NumberBC027599
Gene Symbol IL-5RA
Gene ID (NCBI) 3568
RRIDAB_2127342
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01344
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-5 receptor subunit alpha (IL-5RA), also known as CD125, is a type I transmembrane protein that belongs to the type I cytokine receptor family. IL-5RA is a critical component of the high-affinity IL-5 receptor complex, IL-5R, which consists of the alpha chain (IL-5RA) that specifically binds to IL-5 and the common beta chain (CD131) shared with receptors for IL-3 and GM-CSF (PMID: 1833065; 7872157). IL-5/IL-5RA signaling axis is crucial for the differentiation, proliferation, recruitment, and activation of eosinophils, and is also involved in pathological eosinophilia (PMID: 34638583).

Protocols

Product Specific Protocols
WB protocol for IL-5RA antibody 12655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

J Allergy Clin Immunol

Mepolizumab does not alter levels of eosinophils, T cells, and mast cells in the duodenal mucosa in eosinophilic esophagitis

Authors - Sébastien Conus
human

Front Immunol

NET Formation in Bullous Pemphigoid Patients With Relapse Is Modulated by IL-17 and IL-23 Interplay.

Authors - Delphine Giusti
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