IL-5RA/CD125 Polyclonal antibody

• 

  WB analysis of human peripheral blood platelets using 12655-1-AP

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12655-1-AP (IL-5RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12655-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12655-1-AP (IL5RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

IL-5RA/CD125 Polyclonal Antibody for WB, ELISA

Cat No. 12655-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IL5RA, CD125, HSIL5R3, IL 5 receptor subunit alpha, IL 5R alpha

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Tested Applications

• Positive WB detected in: human peripheral blood platelets, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12655-1-AP targets IL-5RA/CD125 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3358

  Product name: Recombinant human IL-5RA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 50-335 aa of BC027599

  Sequence: WKPNPDQEQRNVNLEYQVKINAPKEDDYETRITESKCVTILHKGFSASVRTILQNDHSLLASSWASAELHAPPGSPGTSIVNLTCTTNTTEDNYSRLRSYQVSLHCTWLVGTDAPEDTQYFLYYRYGSWTEECQEYSKDTLGRNIACWFPRTFILSKGRDWLAVLVDGSSKHSAIRPFDQLFALHAIDQINPPLNVTAEIEGTRLSIQWEKPVSAFPIHCFDYEVKIHNTRNGYLQIEKLMTNAFISIIDDLSKYDVQVRAAVSSMCREAGLWSEWSQPIYVGFSR

• Full Name: interleukin 5 receptor, alpha
• Calculated Molecular Weight: 48 kDa, 38 kDa
• Observed Molecular Weight: 70-75 kDa, 43 kDa
• GenBank Accession Number: BC027599
• Gene Symbol: IL-5RA/CD125
• Gene ID (NCBI): 3568
• RRID: AB_2127342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01344
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-5 receptor subunit alpha (IL-5RA), also known as CD125, is a type I transmembrane protein that belongs to the type I cytokine receptor family. IL-5RA is a critical component of the high-affinity IL-5 receptor complex, IL-5R, which consists of the alpha chain (IL-5RA) that specifically binds to IL-5 and the common beta chain (CD131) shared with receptors for IL-3 and GM-CSF (PMID: 1833065; 7872157). IL-5/IL-5RA signaling axis is crucial for the differentiation, proliferation, recruitment, and activation of eosinophils, and is also involved in pathological eosinophilia (PMID: 34638583).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		J Allergy Clin Immunol; Mepolizumab does not alter levels of eosinophils, T cells, and mast cells in the duodenal mucosa in eosinophilic esophagitis; Authors - Sébastien Conus
human		Front Immunol; NET Formation in Bullous Pemphigoid Patients With Relapse Is Modulated by IL-17 and IL-23 Interplay.; Authors - Delphine Giusti