IMPDH2 Monoclonal antibody

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  WB analysis using 67663-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67663-1-Ig (IMPDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of A549 using 67663-1-Ig

  WB result of IMPDH2 antibody (67663-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMPDH2 transfected A549 cells.

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  WB analysis using 67663-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67663-1-Ig (IMPDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67663-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67663-1-Ig (IMPDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast hyperplasia using 67663-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 67663-1-Ig (IMPDH2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 67663-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 67663-1-Ig (IMPDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 67663-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 67663-1-Ig (IMPDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 67663-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using IMPDH2 antibody (67663-1-Ig, Clone: 1B1G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

IMPDH2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67663-1-Ig

Clone No.1B1G8

Publications(3)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

IMP dehydrogenase 2, IMPD 2, IMPD2, IMPDH 2, IMPDH II, IMPDH2

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Tested Applications

• Positive WB detected in: A549 cells, HSC-T6 cells, 4T1 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, PC-12 cells
• Positive IHC detected in: human breast hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

67663-1-Ig targets IMPDH2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17170

  Product name: Recombinant human IMPDH2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC012840

  Sequence: MADYLISGGTSYVPDDGLTAQQLFNCGDGLTYNDFLILPGYIDFTADQVDLTSALTKKITLKTPLVSSPMDTVTEAGMAIAMALTGGIGFIHHNCTPEFQANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVIGGNVVTAAQAKNLIDAGVDALRVGMGSGSICITQEVLACGRPQATAVYKV

• Full Name: IMP (inosine monophosphate) dehydrogenase 2
• Calculated Molecular Weight: 514 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC012840
• Gene Symbol: IMPDH2
• Gene ID (NCBI): 3615
• RRID: AB_2882860
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12268
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Regulation of local GTP availability controls RAC1 activity and cell invasion; Authors - Anna Bianchi-Smiraglia
human	WB	Epigenomics; Epigenetic silencing of KCTD8 promotes hepatocellular carcinoma growth by activating PI3K/AKT signaling; Authors - Jing Zhou ; KD Validated
human	WB,IHC,IF,CoIP	J Exp Clin Cancer Res; Hypoxia-induced TMTC3 expression in esophageal squamous cell carcinoma potentiates tumor angiogenesis through Rho GTPase/STAT3/VEGFA pathway; Authors - Hongyu Yuan ; KD Validated