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  • KD/KO Validated

IMPDH2 Monoclonal antibody

IMPDH2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1B1G8

Cat no : 67663-1-Ig

Synonyms

IMP dehydrogenase 2, IMPD 2, IMPD2, IMPDH 2, IMPDH II, IMPDH2



Tested Applications

Positive WB detected inA549 cells, HSC-T6 cells, 4T1 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, PC-12 cells
Positive IHC detected inhuman breast hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67663-1-Ig targets IMPDH2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IMPDH2 fusion protein Ag17170
Full Name IMP (inosine monophosphate) dehydrogenase 2
Calculated Molecular Weight 514 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC012840
Gene Symbol IMPDH2
Gene ID (NCBI) 3615
RRIDAB_2882860
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270)

Protocols

Product Specific Protocols
WB protocol for IMPDH2 antibody 67663-1-IgDownload protocol
IHC protocol for IMPDH2 antibody 67663-1-IgDownload protocol
IF protocol for IMPDH2 antibody 67663-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Regulation of local GTP availability controls RAC1 activity and cell invasion

Authors - Anna Bianchi-Smiraglia
humanWB

Epigenomics

Epigenetic silencing of KCTD8 promotes hepatocellular carcinoma growth by activating PI3K/AKT signaling

Authors - Jing Zhou
  • KD Validated
humanWB,IHC,IF,CoIP

J Exp Clin Cancer Res

Hypoxia-induced TMTC3 expression in esophageal squamous cell carcinoma potentiates tumor angiogenesis through Rho GTPase/STAT3/VEGFA pathway

Authors - Hongyu Yuan
  • KD Validated