ING1-specific Polyclonal antibody

ING1-specific Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16185-1-AP

Synonyms

ING1, p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a



Tested Applications

Positive WB detected inhuman heart tissue
Positive IP detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

16185-1-AP targets ING1-specific in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name inhibitor of growth family, member 1
Calculated molecular weight 47 kDa
Observed molecular weight 30 kDa
GenBank accession numberNM_198218
Gene symbol ING1
Gene ID (NCBI) 3621
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING1 is the first identified one, interacting with TP53 and is a component of the p53 signaling pathway. Multiple alternatively spliced transcript variants encoding distinct isoforms of ING1 have been reported. This antibody recognizes all 24/33/47kd isoforms of ING1, but will not bind ING2, 3 or 4.

Protocols

Product Specific Protocols
WB protocol for ING1-specific antibody 16185-1-APDownload protocol
IP protocol for ING1-specific antibody 16185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols