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  • KD/KO Validated

ING3 Polyclonal antibody

ING3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10905-1-AP

Synonyms

Eaf4, ING2, ING3, Inhibitor of growth protein 3, p47ING3



Tested Applications

Positive WB detected inmouse uterus tissue, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10905-1-AP targets ING3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ING3 fusion protein Ag1357
Full Name inhibitor of growth family, member 3
Calculated molecular weight 46 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC009777
Gene symbol ING3
Gene ID (NCBI) 54556
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING3 can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. This antibody is specifically against p47ING3.

Protocols

Product Specific Protocols
WB protocol for ING3 antibody 10905-1-APDownload protocol
IHC protocol for ING3 antibody 10905-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Indian J Med Res

Downregulated inhibitor of growth 3 (ING3) expression during colorectal carcinogenesis.

Authors - Wen-Feng Gou
humanWB

Mol Cancer Res

RSK Promotes Prostate Cancer Progression in Bone through ING3, CKAP2 and PTK6-mediated Cell Survival.

Authors - Guoyu Yu
humanWB

Mol Cell Biol

p90 RSK2 mediates antianoikis signals by both transcription-dependent and -independent mechanisms.

Authors - Jin Lingtao L
humanWB

Oncogene

The tumor suppressor ING3 is degraded by SCF(Skp2)-mediated ubiquitin-proteasome system.

Authors - Chen G G
  • KD Validated
humanIHC

Clin Cancer Res

Prognostic significance of nuclear ING3 expression in human cutaneous melanoma.

Authors - Wang Yemin Y