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ING3 Polyclonal antibody

ING3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10905-1-AP

Synonyms

Eaf4, ING2, ING3, Inhibitor of growth protein 3, p47ING3



Tested Applications

Positive WB detected inmouse uterus tissue, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10905-1-AP targets ING3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ING3 fusion protein Ag1357
Full Name inhibitor of growth family, member 3
Calculated molecular weight 46 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC009777
Gene symbol ING3
Gene ID (NCBI) 54556
RRIDAB_2127780
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING3 can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. This antibody is specifically against p47ING3.

Protocols

Product Specific Protocols
WB protocol for ING3 antibody 10905-1-APDownload protocol
IHC protocol for ING3 antibody 10905-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Clin Cancer Res

Prognostic significance of nuclear ING3 expression in human cutaneous melanoma.

Authors - Wang Yemin Y
humanWB

Oncogene

The tumor suppressor ING3 is degraded by SCF(Skp2)-mediated ubiquitin-proteasome system.

Authors - Chen G G
  • KD Validated
humanWB

Mol Cell Biol

p90 RSK2 mediates antianoikis signals by both transcription-dependent and -independent mechanisms.

Authors - Jin Lingtao L
humanIHC

Front Oncol

Nuclear ING3 Expression Is Correlated With a Good Prognosis of Breast Cancer.

Authors - Xiaoyan Wu
humanWB

Mol Cancer Res

RSK Promotes Prostate Cancer Progression in Bone through ING3, CKAP2 and PTK6-mediated Cell Survival.

Authors - Guoyu Yu
humanWB,IHC

Indian J Med Res

Downregulated inhibitor of growth 3 (ING3) expression during colorectal carcinogenesis.

Authors - Wen-Feng Gou