|Positive WB detected in||mouse uterus tissue, HepG2 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10905-1-AP targets ING3 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ING3 fusion protein Ag1357|
|Full Name||inhibitor of growth family, member 3|
|Calculated molecular weight||46 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC009777|
|Gene ID (NCBI)||54556|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING3 can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. This antibody is specifically against p47ING3.
Indian J Med Res
Downregulated inhibitor of growth 3 (ING3) expression during colorectal carcinogenesis.
Mol Cancer Res
RSK Promotes Prostate Cancer Progression in Bone through ING3, CKAP2 and PTK6-mediated Cell Survival.
Nuclear ING3 Expression Is Correlated With a Good Prognosis of Breast Cancer.
Mol Cell Biol
p90 RSK2 mediates antianoikis signals by both transcription-dependent and -independent mechanisms.
The tumor suppressor ING3 is degraded by SCF(Skp2)-mediated ubiquitin-proteasome system.
Clin Cancer Res
Prognostic significance of nuclear ING3 expression in human cutaneous melanoma.