ING3-specific Polyclonal antibody

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  WB analysis of human kidney using 16187-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16187-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16187-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16187-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16187-1-AP

  IP result of anti-ING3-specific (IP:16187-1-AP, 4ug; Detection:16187-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

ING3-specific Polyclonal Antibody for WB, IP, ELISA

Cat No. 16187-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

ING3, Eaf4, HSPC301, ING2, Inhibitor of growth protein 3

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Tested Applications

• Positive WB detected in: human kidney tissue, mouse heart tissue, mouse kidney tissue, mouse liver tissue
• Positive IP detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16187-1-AP targets ING3-specific in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: inhibitor of growth family, member 3
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: NM_019071
• Gene Symbol: ING3
• Gene ID (NCBI): 54556
• RRID: AB_2264993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NXR8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING3 can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. This antibody is specifically against p47ING3.