ING4-specific Polyclonal antibody

ING4-specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16188-1-AP

Synonyms

ING4, Inhibitor of growth protein 4, my036, p29ING4



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, mouse colon tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16188-1-AP targets ING4-specific in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name inhibitor of growth family, member 4
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29-32 kDa
GenBank Accession NumberNM_001127582
Gene Symbol ING4
Gene ID (NCBI) 51147
RRIDAB_2127798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ING4, also named as p29ING4, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. ING4 can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. It may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). ING4 is a tumor suppressor gene that interacts with NFkB and represses its transcriptional activity. Several lines of evidence suggest that the tumor suppressor gene ING4, NFkB and its target genes matrix metalloproteases MMP-2, MMP-9 and u-PA are critically involved in tumor invasion. This antibody is specifically against p29ING4.

Protocols

Product Specific Protocols
WB protocol for ING4-specific antibody 16188-1-APDownload protocol
IHC protocol for ING4-specific antibody 16188-1-APDownload protocol
IF protocol for ING4-specific antibody 16188-1-APDownload protocol
IP protocol for ING4-specific antibody 16188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

ING4 regulates a secretory phenotype in primary fibroblasts with dual effects on cell proliferation and tumor growth.

Authors - Moreno A A
humanIHC

PLoS One

Novel multiple markers to distinguish melanoma from dysplastic nevi.

Authors - Zhang Guohong G

Am J Physiol Heart Circ Physiol

Carvedilol-responsive microRNAs, miR-199a-3p and -214 protect cardiomyocytes from simulated ischemia-reperfusion injury.

Authors - Kyoung-Mi Park
humanWB,IHC,IF

Oral Oncol

Downregulation and translocation of nuclear ING4 is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma.

Authors - Li Xiao-han XH
humanIHC

Acta Histochem

Inhibitor of growth 4 (ING4) inhibits hypoxia-induced EMT by decreasing HIF-1α and snail in HK2 cells.

Authors - Lingling Lu
humanIHC

Mol Med Rep

PMID: 27484725

Authors - Xin Ren