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  • KD/KO Validated

INPP5E Polyclonal antibody

INPP5E Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17797-1-AP

Synonyms

INPP5E, PPI5PIV



Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells, mouse brain tissue, mouse testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman testis tissue, human kidney tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17797-1-AP targets INPP5E in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP5E fusion protein Ag11959
Full Name inositol polyphosphate-5-phosphatase, 72 kDa
Calculated molecular weight 644 aa, 70 kDa
Observed molecular weight 64-66 kDa
GenBank accession numberBC028032
Gene symbol INPP5E
Gene ID (NCBI) 56623
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.

Protocols

Product Specific Protocols
WB protocol for INPP5E antibody 17797-1-APDownload protocol
IHC protocol for INPP5E antibody 17797-1-APDownload protocol
IF protocol for INPP5E antibody 17797-1-APDownload protocol
IP protocol for INPP5E antibody 17797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Adv

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Authors - Chuan Chen
  • KO Validated
humanIF

Nat Commun

Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.

Authors - Qingwen Xu

Nat Commun

KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling.

Authors - Kenneth B Schou
mouse, humanWB,IF

Proc Natl Acad Sci U S A

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Authors - Humbert Melissa C MC
  • KD Validated
humanIF

Curr Biol

Exocyst complex mediates recycling of internal cilia.

Authors - Félix E Rivera-Molina
mouseWB

JCI Insight

Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling.

Authors - Hairong Shi