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INPP5E Polyclonal antibody
INPP5E Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 17797-1-AP
Validation Data Gallery
|Positive WB detected in||SH-SY5Y cells, HEK-293 cells, mouse brain tissue, mouse testis tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human testis tissue, human kidney tissue, human placenta tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE1 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17797-1-AP targets INPP5E in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||INPP5E fusion protein Ag11959|
|Full Name||inositol polyphosphate-5-phosphatase, 72 kDa|
|Calculated molecular weight||644 aa, 70 kDa|
|Observed molecular weight||64-66 kDa|
|GenBank accession number||BC028032|
|Gene ID (NCBI)||56623|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.
|Product Specific Protocols|
|WB protocol for INPP5E antibody 17797-1-AP||Download protocol|
|IHC protocol for INPP5E antibody 17797-1-AP||Download protocol|
|IF protocol for INPP5E antibody 17797-1-AP||Download protocol|
|IP protocol for INPP5E antibody 17797-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.
Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.
KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling.
Proc Natl Acad Sci U S A
ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.
Exocyst complex mediates recycling of internal cilia.
Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Charlotte (Verified Customer) (07-24-2023)
INPP5E stained in FITC is compared to ARL13b in Cy3. Methanol fixation, Block 1h, incubate 2h with primary ab, and 1h with secondary ab. The antibody is still a bit noisy (despite the blocking at 5% and ab diluted in 2%BSA) but the signal is strong and match what we expect.