INSIG1 Polyclonal antibody

INSIG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55282-1-AP

Synonyms

CL 6, INSIG 1, INSIG1, insulin induced gene 1, Insulin induced gene 1 protein



Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55282-1-AP targets INSIG1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name insulin induced gene 1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberNM_005542
Gene Symbol INSIG1
Gene ID (NCBI) 3638
RRIDAB_2881298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for INSIG1 antibody 55282-1-APDownload protocol
IHC protocol for INSIG1 antibody 55282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Acacetin protects against depression-associated dry eye disease by regulating ubiquitination of NLRP3 through gp78 signal

Authors - Mingxia Xie
chickenWB,IF

Poult Sci

Functional and miRNA regulatory characteristics of INSIG genes highlight the key role of lipid synthesis in the liver of chicken (Gallus gallus)

Authors - Yaxin Yue
human,mouseWB,IF

FASEB J

Dickkopf-1 promotes vascular smooth muscle cell foam cell formation and atherosclerosis development through CYP4A11/SREBP2/ABCA1

Authors - Xiao Li
human,mouseWB

Metabolism

miR-32-5p induces hepatic steatosis and hyperlipidemia by triggering de novo lipogenesis

Authors - Ya-Di Wang
humanWB

Redox Biol

Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis

Authors - Xue Yang
ratWB

Cell Death Differ

C9orf72 controls hepatic lipid metabolism by regulating SREBP1 transport

Authors - Yachen Wu