INVS Polyclonal antibody

• 

  WB analysis of MCF-7 using 10585-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10585-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10585-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10585-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10585-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10585-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10585-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10585-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10585-1-AP (INVS antibody) at dilution of 1:100 (under 25x lens).

• 

  IHC staining of human breast cancer using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10585-1-AP (INVS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10585-1-AP (INVS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10585-1-AP (INVS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 10585-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10585-1-AP (INVS antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of hTERT-RPE1 cells using 10585-1-AP

  IF result from Dr. Corbit, Kevin. anti-INVS (also named as NPHP2; 10585-1-AP) marks the ‘inversin compartment’ and basal bodies of Human hTERT-RPE1 cells.

• 

  IF Staining of HeLa using 10585-1-AP

  Immunofluorescent analysis of Hela cells, using INVS antibody 10585-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

INVS Polyclonal Antibody for WB, IF, IHC, ELISA

Cat No. 10585-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Synonyms

INV, inversin, INVS, KIAA0573, Nephrocystin 2

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Tested Applications

• Positive WB detected in: MCF-7 cells, K-562 cells, L02 cells, SH-SY5Y cells, mouse skeletal muscle tissue, HepG2 cells, Raji cells, Jurkat cells
• Positive IHC detected in: human breast cancer tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells, hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Product Information

10585-1-AP targets INVS in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0934

  Product name: Recombinant human INVS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-101 aa of BC006370

  Sequence: MNKSENLLFAGSSLASQVHAAAVNGDKGALQRLIVGNSALKDKEDQFGRTPLMYCVLADRLDCADALLKAGADVNKTDHSQRTALHLAAQKALRTISTGRI

• Full Name: inversin
• Calculated Molecular Weight: 118 kDa, 100 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC006370
• Gene Symbol: INVS
• Gene ID (NCBI): 27130
• RRID: AB_2233902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y283
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INVS, short for inversin, plays a role in primary cilia function and involvement in the cell cycle. It is required for normal renal development and establishment of the left-right axis. INVS may be involved in Wnt signaling pathways by acting as a molecular switch between different them. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. This antibody is a rabbit polyclonal antibody raised against part of the N-terminus of human INVS.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	EMBO J; Cell type-specific regulation of ciliary transition zone assembly in vertebrates.; Authors - Antonia Wiegering ; KO Validated
	IF	Proc Natl Acad Sci U S A; A complement factor H homolog, heparan sulfation, and syndecan maintain inversin compartment boundaries in C. elegans cilia.; Authors - Natalie Acker
rat	WB	Endocrinology; PCP protein inversin regulates testis function through changes in cytoskeletal organization of actin and microtubules.; Authors - Linxi Li ; KD Validated
rat	IHC	Virchows Arch; Afferent arteriolopathy and glomerular collapse but not segmental sclerosis induce tubular atrophy in old spontaneously hypertensive rats.; Authors - Leh Sabine S
human	IF	Am J Med Genet A; Early presentation of cystic kidneys in a family with a homozygous INVS mutation.; Authors - Machteld M Oud
human	FC	JCI Insight; Cilia proteins are biomarkers of altered flow in the vasculature.; Authors - Ankan Gupta