- Featured Product
- KD/KO Validated
INVS Polyclonal antibody
INVS Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, IF, FC, ELISA
Cat no : 10585-1-AP
Validation Data Gallery
|Positive WB detected in||MCF-7 cells, K-562 cells, L02 cells, SH-SY5Y cells, mouse skeletal muscle tissue, HepG2 cells, Raji cells, Jurkat cells|
|Positive IHC detected in||human breast cancer tissue, human hepatocirrhosis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells, hTERT-RPE1 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 3 publications below|
|IHC||See 1 publications below|
|IF||See 3 publications below|
|FC||See 1 publications below|
10585-1-AP targets INVS in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||INVS fusion protein Ag0934|
|Calculated molecular weight||118 kDa, 100 kDa|
|Observed molecular weight||100-110 kDa|
|GenBank accession number||BC006370|
|Gene ID (NCBI)||27130|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
INVS, short for inversin, plays a role in primary cilia function and involvement in the cell cycle. It is required for normal renal development and establishment of left-right axis. INVS may involve in Wnt signaling pathways by acting as a molecular switch between different them. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. This antibody is a rabbit polyclonal antibody raised against part of N-terminus of human INVS. The calculated molecular weight of INVS is 80 kDa, but the modified INVS protein is about 100-110 kDa.
|Product Specific Protocols|
|WB protocol for INVS antibody 10585-1-AP||Download protocol|
|IHC protocol for INVS antibody 10585-1-AP||Download protocol|
|IF protocol for INVS antibody 10585-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell type-specific regulation of ciliary transition zone assembly in vertebrates.
Proc Natl Acad Sci U S A
A complement factor H homolog, heparan sulfation, and syndecan maintain inversin compartment boundaries in C. elegans cilia.
PCP protein inversin regulates testis function through changes in cytoskeletal organization of actin and microtubules.
Afferent arteriolopathy and glomerular collapse but not segmental sclerosis induce tubular atrophy in old spontaneously hypertensive rats.
Am J Med Genet A
Early presentation of cystic kidneys in a family with a homozygous INVS mutation.
Cilia proteins are biomarkers of altered flow in the vasculature.