IPPK Polyclonal antibody

• Featured Product
• KD/KO Validated

IPPK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12603-1-AP

Synonyms

C9orf12, Ins(1,3,4,5,6)P5 2 kinase, InsP5 2 kinase, INSP5K2, IP5K, IPK1, IPK1 homolog, IPPK, KIAA0699

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Validation Data Gallery

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  WB analysis of Jurkat using 12603-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12603-1-AP (IPPK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12603-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12603-1-AP (IPPK antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

12603-1-AP targets IPPK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IPPK fusion protein Ag3278
• Full Name: inositol 1,3,4,5,6-pentakisphosphate 2-kinase
• Calculated Molecular Weight: 491 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC026154
• Gene Symbol: IPPK
• Gene ID (NCBI): 64768
• RRID: AB_2127848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Inositol hexakisphosphate (IP6) generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function.; Authors - Paul C Scherer ; KD Validated