IQCK Polyclonal antibody

IQCK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25740-1-AP

Synonyms

IQ domain containing protein K, IQ motif containing K, IQCK

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Validation Data Gallery

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  WB analysis of mouse testis using 25740-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25740-1-AP (IQCK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 25740-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25740-1-AP (IQCK Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 25740-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25740-1-AP (IQCK Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 25740-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25740-1-AP (IQCK Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 25740-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25740-1-AP (IQCK Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of COLO 320 using 25740-1-AP

  Immunofluorescent analysis of COLO 320 cells using 25740-1-AP (IQCK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human colon cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25740-1-AP is IQCK Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IQCK fusion protein Ag22680
• Full Name: IQ motif containing K
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC034823
• Gene Symbol: IQCK
• Gene ID (NCBI): 124152
• RRID: AB_2880220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	IHC,WB,IF	Front Cell Neurosci; Novel Alzheimer risk factor IQ motif containing protein K is abundantly expressed in the brain and is markedly increased in patients with Alzheimer's disease.; Authors - Hongjie Wang