MUM1/IRF4 Polyclonal antibody

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  WB analysis using 11247-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 11247-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 11247-2-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11247-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11247-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11247-2-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 11247-2-AP

  IP result of anti-MUM1/IRF4 (IP:11247-2-AP, 3ug; Detection:11247-2-AP 1:500) with mouse spleen tissue lysate 4000ug.

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  IHC staining of human thymus using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of RAW 264.7 using 11247-2-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using MUM1/IRF4 antibody (11247-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

MUM1/IRF4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11247-2-AP

Publications(20)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

IRF4, interferon regulatory factor 4, IRF 4, IRF-4, LSIRF

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Tested Applications

• Positive WB detected in: mouse spleen tissue, mouse lung tissue, mouse thymus tissue, Raji cells, RAW264.7, rat spleen tissue
• Positive IP detected in: mouse spleen tissue
• Positive IHC detected in: human thymus tissue, human lymphoma tissue, human tonsillitis tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Product Information

11247-2-AP targets MUM1/IRF4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1762

  Product name: Recombinant human IRF4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-451 aa of BC015752

  Sequence: MNLEGGGRGGEFGMSAVSCGNGKLRQWLIDQIDSGKYPGLVWENEEKSIFRIPWKHAGKQDYNREEDAALFKAWALFKGKFREGIDKPDPPTWKTRLRCALNKSNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPPLDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGPACENGCQVTGTFYACAPPESQAPGVPTEPSIRSAEALAFSDCRLHICLYYREILVKELTTSSPEGCRISHGHTYDASNLDQVLFPYPEDNGQRKNIEKLLSHLERGVVLWMAPDGLYAKRLCQSRIYWDGPLALCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE

• Full Name: IRF 4
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC015752
• Gene Symbol: IRF4
• Gene ID (NCBI): 3662
• ENSEMBL Gene ID: ENSG00000137265
• RRID: AB_2264940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15306
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Cancer Commun (Lond); Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies; Authors - Lu Tang
mouse,human	WB	Diabetes; Obese Skeletal Muscle-Expressed Interferon Regulatory Factor 4 Transcriptionally Regulates Mitochondrial Branched-Chain Aminotransferase Reprogramming Metabolome.; Authors - Ting Yao ; KD Validated
mouse	WB	Nanotoxicology; Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis.; Authors - Jie Dong
human	IHC	Diabetes Res Clin Pract; fMaggot treatment promotes healing of diabetic foot ulcer wounds possibly by upregulating Treg levels.; Authors - Jie Zhang
mouse	IHC	Int Immunopharmacol; IRF-4 deficiency reduces inflammation and kidney fibrosis after folic acid-induced acute kidney injury.; Authors - Meixin Chen ; KO Validated
mouse	WB,IHC	Mediators Inflamm; MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression.; Authors - Aijun Jia

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shunbin (Verified Customer) (05-01-2023)

One of the best antibody we have used for western blotting. 1:10,000 produces a single specific band. original concentration: 440 ug/ml

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: B/T cell lines

MUM1/IRF4 Antibody Western Blot validation (1:10,000 dilution) in B/T cell lines (Cat no:11247-2-AP)