MUM1/IRF4 Polyclonal antibody

MUM1/IRF4 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 11247-2-AP

Synonyms

IRF 4, IRF4, LSIRF, Multiple myeloma oncogene 1, MUM1, NF EM5



Tested Applications

Positive WB detected inmouse spleen tissue, Jurkat cells, mouse lung tissue, mouse thymus tissue, Raji cells
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman thymus tissue, human lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11247-2-AP targets MUM1/IRF4 in WB, IP, IHC, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUM1/IRF4 fusion protein Ag1762
Full Name IRF 4
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC015752
Gene symbol IRF4
Gene ID (NCBI) 3662
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

Protocols

Product Specific Protocols
WB protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
IHC protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
IP protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
FC protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Adv Sci (Weinh)

IRF4 in Skeletal Muscle Regulates Exercise Capacity via PTG/Glycogen Pathway.

Authors - Xiaopeng Zhu
mouseWB,IHC

Mediators Inflamm

MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression.

Authors - Aijun Jia
mouseWB

Nanotoxicology

Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis.

Authors - Jie Dong