|Positive WB detected in||mouse spleen tissue, Jurkat cells, mouse lung tissue, mouse thymus tissue, Raji cells|
|Positive IP detected in||mouse spleen tissue|
|Positive IHC detected in||human thymus tissue, human lymphoma tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Raji cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11247-2-AP targets MUM1/IRF4 in WB, IP, IHC, FC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MUM1/IRF4 fusion protein Ag1762|
|Full Name||IRF 4|
|Calculated molecular weight||52 kDa|
|Observed molecular weight||52 kDa|
|GenBank accession number||BC015752|
|Gene ID (NCBI)||3662|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
|Product Specific Protocols|
|WB protocol for MUM1/IRF4 antibody 11247-2-AP||Download protocol|
|IHC protocol for MUM1/IRF4 antibody 11247-2-AP||Download protocol|
|IP protocol for MUM1/IRF4 antibody 11247-2-AP||Download protocol|
|FC protocol for MUM1/IRF4 antibody 11247-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Adv Sci (Weinh)
IRF4 in Skeletal Muscle Regulates Exercise Capacity via PTG/Glycogen Pathway.
MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression.
Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis.