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  • KD/KO Validated

MUM1/IRF4 Polyclonal antibody

MUM1/IRF4 Polyclonal Antibody for WB, IP, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 11247-2-AP


IRF 4, IRF4, LSIRF, Multiple myeloma oncogene 1, MUM1, MUM1/IRF4, NF EM5

Tested Applications

Positive WB detected inmouse spleen tissue, Jurkat cells, mouse thymus tissue, Raji cells, RAW264.7, mouse lung tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman thymus tissue, human lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRamos cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11247-2-AP targets MUM1/IRF4 in WB, IP, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUM1/IRF4 fusion protein Ag1762
Full Name IRF 4
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015752
Gene Symbol IRF4
Gene ID (NCBI) 3662
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.


Product Specific Protocols
WB protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
IHC protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
IP protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Obese Skeletal Muscle-Expressed Interferon Regulatory Factor 4 Transcriptionally Regulates Mitochondrial Branched-Chain Aminotransferase Reprogramming Metabolome.

Authors - Ting Yao
  • KD Validated


Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis.

Authors - Jie Dong

Diabetes Res Clin Pract

fMaggot treatment promotes healing of diabetic foot ulcer wounds possibly by upregulating Treg levels.

Authors - Jie Zhang

Int Immunopharmacol

IRF-4 deficiency reduces inflammation and kidney fibrosis after folic acid-induced acute kidney injury.

Authors - Meixin Chen
  • KO Validated

Mediators Inflamm

MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression.

Authors - Aijun Jia

Adv Sci (Weinh)

IRF4 in Skeletal Muscle Regulates Exercise Capacity via PTG/Glycogen Pathway.

Authors - Xiaopeng Zhu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Shunbin (Verified Customer) (05-01-2023)

One of the best antibody we have used for western blotting. 1:10,000 produces a single specific band. original concentration: 440 ug/ml

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: B/T cell lines
MUM1/IRF4 Antibody Western Blot validation (1:10,000 dilution) in B/T cell lines (Cat no:11247-2-AP)