Validation Data Gallery
|Positive WB detected in||mouse spleen tissue, Jurkat cells, mouse lung tissue, mouse thymus tissue, Raji cells|
|Positive IP detected in||mouse spleen tissue|
|Positive IHC detected in||human thymus tissue, human lymphoma tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Raji cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11247-2-AP targets MUM1/IRF4 in WB, IP, IHC, FC applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MUM1/IRF4 fusion protein Ag1762|
|Full Name||IRF 4|
|Calculated molecular weight||52 kDa|
|Observed molecular weight||52 kDa|
|GenBank accession number||BC015752|
|Gene ID (NCBI)||3662|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
|Product Specific Protocols|
|WB protocol for MUM1/IRF4 antibody 11247-2-AP||Download protocol|
|IHC protocol for MUM1/IRF4 antibody 11247-2-AP||Download protocol|
|IP protocol for MUM1/IRF4 antibody 11247-2-AP||Download protocol|
|FC protocol for MUM1/IRF4 antibody 11247-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Adv Sci (Weinh)
IRF4 in Skeletal Muscle Regulates Exercise Capacity via PTG/Glycogen Pathway.
MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression.
IRF-4 deficiency reduces inflammation and kidney fibrosis after folic acid-induced acute kidney injury.
Diabetes Res Clin Pract
fMaggot treatment promotes healing of diabetic foot ulcer wounds possibly by upregulating Treg levels.
Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis.