Tested Applications
| Positive WB detected in | mouse spleen tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18977-1-AP targets IRF8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ICSBP1 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | NM_002163 |
| Gene Symbol | IRF8 |
| Gene ID (NCBI) | 3394 |
| RRID | AB_10596482 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02556 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IRF8 also named as ICSBP1, is a transcription factor of the IFN regulatory factor (IRF) family. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the IFN consensus sequence (ICS)). It plays a negative regulatory role in cells of the immune system. It is predominantly in lymphoid tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IRF8 antibody 18977-1-AP | Download protocol |
| WB protocol for IRF8 antibody 18977-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-IRF8 antibody (Cat# 18977-1-AP) has 22 total citations published across 21 journals, including Cell Death Dis, J Exp Clin Cancer Res, J Autoimmun, Life Sci, Commun Biol, Eur J Immunol, and Sci Rep. Research fields span oncology (pancreatic cancer, melanoma, lung adenocarcinoma, leukemia), autoimmune diseases (SLE, lupus nephritis, Sjögren's syndrome), inflammation (gastritis, colitis, neuroinflammation), bone biology, and immune regulation.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Musashi2 promotes EGF-induced EMT in pancreatic cancer via ZEB1-ERK/MAPK signaling | ||
Cell Death Dis TRIM63/IRF-8 axis promotes tumor progression and immunosuppression of melanoma with BRAF mutation.
| ||
J Pineal Res Melatonin Inhibits Antibody-Secreting Cells and Improves Systemic Lupus Erythematosus via Modulation of the PKA-CREB Signaling Pathway. | ||
J Autoimmun TIGIT-Fc fusion protein alleviates murine lupus nephritis through the regulation of SPI-B-PAX5-XBP1 axis-mediated B-cell differentiation | ||
Life Sci Mechanism of berberine in treating Helicobacter pylori induced chronic atrophic gastritis through IRF8-IFN-γ signaling axis suppressing. | ||
Life Sci Berberine regulates macrophage polarization through IL-4-STAT6 signaling pathway in Helicobacter pylori-induced chronic atrophic gastritis. |
