IRF8 Polyclonal antibody

IRF8 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18977-1-AP

Synonyms

H ICSBP, ICSBP, ICSBP1, IRF 8, IRF8



Tested Applications

Positive WB detected inmouse spleen tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

18977-1-AP targets IRF8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ICSBP1
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberNM_002163
Gene symbol IRF8
Gene ID (NCBI) 3394
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF8 also named as ICSBP1, is a transcription factor of the IFN regulatory factor (IRF) family. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the IFN consensus sequence (ICS)). It plays a negative regulatory role in cells of the immune system. It is predominantly in lymphoid tissues.

Protocols

Product Specific Protocols
WB protocol for IRF8 antibody 18977-1-APDownload protocol
IHC protocol for IRF8 antibody 18977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Pharm Biol

Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl4/2-AAF.

Authors - Ying Xu
mouseWB

Environ Toxicol

Resveratrol alleviates aluminum-induced intestinal barrier dysfunction in mice.

Authors - Wudi Hao
humanWB,IHC

Life Sci

Mechanism of berberine in treating Helicobacter pylori induced chronic atrophic gastritis through IRF8-IFN-γ signaling axis suppressing.

Authors - Tao Yang
mouseWB

Life Sci

Berberine regulates macrophage polarization through IL-4-STAT6 signaling pathway in Helicobacter pylori-induced chronic atrophic gastritis.

Authors - Tao Yang
humanIHC

Transl Lung Cancer Res

Cross-talk of pyroptosis and tumor immune landscape in lung adenocarcinoma.

Authors - Xin Wang
mouseWB,IF

Mater Sci Eng C Mater Biol Appl

The unique regulation of implant surface nanostructure on macrophages M1 polarization.

Authors - Yide He