IRF9 Antibody

IRF9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14167-1-AP

Synonyms

interferon regulatory factor 9, IRF 9, IRF9, ISGF 3 gamma, ISGF3, ISGF3 p48 subunit, ISGF3G, p48



Tested Applications

Positive WB detected inA549 cells, mouse heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14167-1-AP targets IRF9 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF9 fusion protein Ag5365
Full Name interferon regulatory factor 9
Calculated molecular weight 393aa,44 kDa
Observed molecular weight 44-48 kDa
GenBank accession number BC035716
Gene symbol IRF9
Gene ID (NCBI) 10379
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interferon regulatory factor 9 (IRF9) also named Interferon-stimulated gene factor 3 gamma (ISGF3) is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Protocols

Product Specific Protocols
WB protocol for IRF9 antibody 14167-1-APDownload protocol
IHC protocol for IRF9 antibody 14167-1-APDownload protocol
IF protocol for IRF9 antibody 14167-1-APDownload protocol
IP protocol for IRF9 antibody 14167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse, ratWB,IF

Environ Toxicol Pharmacol

Dioscin alleviates dimethylnitrosamine-induced acute liver injury through regulating apoptosis, oxidative stress and inflammation.

Authors - Weixin Zhang
mouseIHC

PLoS One

Resistance to oncolytic myxoma virus therapy in nf1(-/-)/trp53(-/-) syngeneic mouse glioma models is independent of anti-viral type-I interferon.

Authors - Zemp Franz J FJ
humanWB

Mol Cancer Res

STAT1 and NF-κB Inhibitors Diminish Basal Interferon-Stimulated Gene Expression and Improve the Productive Infection of Oncolytic HSV in MPNST Cells.

Authors - Joshua D Jackson
mouseWB

J Biol Chem

Interferon γ (IFNγ)-Signaling via mTORC2 and Regulatory Effects in the Generation of Type II IFN Biological Responses.

Authors - Barbara Kroczynska
humanWB

Mol Cancer Ther

JAK2 inhibitor SAR302503 abrogates PD-L1 expression and targets therapy resistant non-small cell lung cancers.

Authors - Sean Pitroda

Front Immunol

STAT3 Cooperates With Phospholipid Scramblase 2 to Suppress Type I Interferon Response.

Authors - Ming-Hsun Tsai