ISG15 Polyclonal antibody

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  WB analysis using 15981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15981-1-AP

  Untreated and IFN alpha treated A459 cells, untreated and IFN alpha treated HepG2 cells were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15981-1-AP

  WB result of ISG15 antibody (15981-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ISG15 transfected MCF-7 cells.

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  WB analysis of human brain using 15981-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15981-1-AP (ISG15 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15981-1-AP (ISG15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15981-1-AP (ISG15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15981-1-AP (ISG15 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse brain using 15981-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ISG15 antibody (15981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 15981-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ISG15 antibody (15981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 15981-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15981-1-AP (ISG15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

ISG15 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 15981-1-AP

Publications(74)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA

Synonyms

G1P2, hUCRP, IFI15, Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, A549 cells, MCF-7 cells, PC-3 cells, IFN alpha treated A459 cells, HepG2 cells, IFN alpha treated HepG2 cells
• Positive IHC detected in: human lung cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 59 publications below
• IHC: See 12 publications below
• IF: See 10 publications below
• IP: See 4 publications below
• CoIP: See 3 publications below
• RIP: See 1 publications below

Product Information

15981-1-AP targets ISG15 in WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig, monkey, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ISG15 fusion protein Ag8782
• Full Name: ISG15 ubiquitin-like modifier
• Calculated Molecular Weight: 165 aa, 18 kDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC009507
• Gene Symbol: ISG15
• Gene ID (NCBI): 9636
• RRID: AB_2126302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05161
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
mouse,human	WB,IP,IHC	Exp Mol Med; SIRT1 ISGylation accelerates tumor progression by unleashing SIRT1 from the inactive state to promote its deacetylase activity; Authors - Ji An Kang
human	IF	Nat Commun; Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease.; Authors - Marta Olah
human	WB	Nat Commun; ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins.; Authors - Carolina Villarroya-Beltri
human	WB,IHC,IF,CoIP	J Exp Clin Cancer Res; ISG15 and ISGylation modulates cancer stem cell-like characteristics in promoting tumor growth of anaplastic thyroid carcinoma; Authors - Tong Xu ; KD Validated
human	WB	Cancer Lett; Induction of IFIT1/IFIT3 and inhibition of Bcl-2 orchestrate the treatment of myeloma and leukemia via pyroptosis; Authors - Yuanming He

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tinne (Verified Customer) (06-27-2023)

This antibody worked really well for detecting an up regulation of ISG15 in IF. Highly recommend!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human hippocampal progenitor cells

FH

Chaohui (Verified Customer) (10-19-2021)

The antibody shows a high quality of free ISG15 and conjugated ISG15 spots

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, THP1