ISG15 Polyclonal antibody

• Featured Product
• KD/KO Validated

ISG15 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

33

Reviews (1)

Cat no : 15981-1-AP

Synonyms

G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP

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Validation Data Gallery

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  WB analysis using 15981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15981-1-AP

  Untreated and IFN alpha treated A459 cells, untreated and IFN alpha treated HepG2 cells were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15981-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15981-1-AP

  WB result of ISG15 antibody (15981-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ISG15 transfected MCF-7 cells.

• 

  WB analysis using 15981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15981-1-AP (ISG15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15981-1-AP

  IP Result of anti-ISG15 (IP:15981-1-AP, 4ug; Detection:15981-1-AP 1:500) with HeLa cells lysate 3200ug.

• 

  IHC staining of human lung cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15981-1-AP (ISG15 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15981-1-AP (ISG15 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15981-1-AP (ISG15 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 15981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15981-1-AP (ISG15 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse brain using 15981-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ISG15 antibody (15981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 15981-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ISG15 antibody (15981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15981-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15981-1-AP (ISG15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, human brain tissue, MCF-7 cells, IFN alpha treated A459 cells, HepG2 cells, IFN alpha treated HepG2 cells, PC-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 7 publications below
• WB: See 30 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

15981-1-AP targets ISG15 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ISG15 fusion protein Ag8782
• Full Name: ISG15 ubiquitin-like modifier
• Calculated molecular weight: 165 aa, 18 kDa
• Observed molecular weight: 15-17 kDa
• GenBank accession number: BC009507
• Gene symbol: ISG15
• Gene ID (NCBI): 9636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.

Publications

Species	Application	Title
human	IF	Nat Commun; Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease.; Authors - Marta Olah
human	WB	Nat Commun; ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins.; Authors - Carolina Villarroya-Beltri
human	WB, IHC, IF	Cancer Res; ISG15 is a critical microenvironmental factor for pancreatic cancer stem cells.; Authors - Bruno Sainz
human	WB	Elife; Ribosome biogenesis restricts innate immune responses to virus infection and DNA.; Authors - Christopher Bianco
human	WB	EMBO Rep; Degradation of WTAP blocks antiviral responses by reducing the m 6 A levels of IRF3 and IFNAR1 mRNA; Authors - Yong Ge
human	WB	MBio; ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.; Authors - Edmund Osei Kuffour

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chaohui (Verified Customer) (10-19-2021)

The antibody shows a high quality of free ISG15 and conjugated ISG15 spots

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, THP1

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