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ISG15 Polyclonal antibody

ISG15 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15981-1-AP

Synonyms

G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP



Tested Applications

Positive WB detected inHeLa cells, A549 cells, human brain tissue, MCF-7 cells, IFN alpha treated A459 cells, HepG2 cells, IFN alpha treated HepG2 cells, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15981-1-AP targets ISG15 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ISG15 fusion protein Ag8782
Full Name ISG15 ubiquitin-like modifier
Calculated molecular weight 165 aa, 18 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberBC009507
Gene symbol ISG15
Gene ID (NCBI) 9636
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.

Protocols

Product Specific Protocols
WB protocol for ISG15 antibody 15981-1-APDownload protocol
IHC protocol for ISG15 antibody 15981-1-APDownload protocol
IF protocol for ISG15 antibody 15981-1-APDownload protocol
IP protocol for ISG15 antibody 15981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease.

Authors - Marta Olah
humanWB

Nat Commun

ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins.

Authors - Carolina Villarroya-Beltri
humanWB, IHC, IF

Cancer Res

ISG15 is a critical microenvironmental factor for pancreatic cancer stem cells.

Authors - Bruno Sainz
humanWB

Elife

Ribosome biogenesis restricts innate immune responses to virus infection and DNA.

Authors - Christopher Bianco
humanWB

EMBO Rep

Degradation of WTAP blocks antiviral responses by reducing the m 6 A levels of IRF3 and IFNAR1 mRNA

Authors - Yong Ge
humanWB

MBio

ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.

Authors - Edmund Osei Kuffour

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chaohui (Verified Customer) (10-19-2021)

The antibody shows a high quality of free ISG15 and conjugated ISG15 spots

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293, THP1