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  • KD/KO Validated

ISG15 Polyclonal antibody

ISG15 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15981-1-AP

Synonyms

G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP



Tested Applications

Positive WB detected inhuman brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15981-1-AP targets ISG15 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity goat, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ISG15 fusion protein Ag8782
Full Name ISG15 ubiquitin-like modifier
Calculated molecular weight 165 aa, 18 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberBC009507
Gene symbol ISG15
Gene ID (NCBI) 9636
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.

Protocols

Product Specific Protocols
WB protocol for ISG15 antibody 15981-1-APDownload protocol
IHC protocol for ISG15 antibody 15981-1-APDownload protocol
IF protocol for ISG15 antibody 15981-1-APDownload protocol
IP protocol for ISG15 antibody 15981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

The histone chaperone HIRA promotes the induction of host innate immune defences in response to HSV-1 infection.

Authors - Steven McFarlane
humanIP

mBio

ISG15 Connects Autophagy and IFN-γ-Dependent Control of Toxoplasma gondii Infection in Human Cells.

Authors - Jaya Bhushan
  • KO Validated
goatWB

Res Vet Sci

Infection of goats with goatpox virus triggers host antiviral defense through activation of innate immune signaling.

Authors - Xiancheng Zeng
humanWB

Pathol Oncol Res

The Overexpression of CD80 and ISG15 Are Associated with the Progression and Metastasis of Breast Cancer by a Meta-Analysis Integrating Three Microarray Datasets.

Authors - Yuanhang Li
humanWB

Biochem Biophys Res Commun

PMID: 24735544

Authors - Wei Zhang
WB

PLoS One

Determining the molecular drivers of species-specific interferon-stimulated gene product 15 interactions with nairovirus ovarian tumor domain proteases.

Authors - John V Dzimianski