CD11c/Integrin alpha X Antibody

CD11c/Integrin alpha X Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2F1C10

Cat no : 60258-1-Ig

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6



Tested Applications

Positive WB detected inhuman spleen tissue, THP-1 cells, HL-60 cells, U-937 cells
Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue, human breast cancer tissue, human appendicitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Sample-dependent, check data in validation data gallery

Product Information

The 60258-1-Ig immunogen is Fusion Protein CD11c/Integrin alpha X protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD11c/Integrin alpha X fusion protein Ag11350
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated molecular weight 1169aa,129 kDa
Observed molecular weight 145 kDa
GenBank accession number BC038237
Gene symbol ITGAX
Gene ID (NCBI) 3687
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

Protocols

Product Specific Protocols
WB protocol for CD11c/Integrin alpha X antibody 60258-1-IgDownload protocol
IHC protocol for CD11c/Integrin alpha X antibody 60258-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIHC

PLoS One

Food hypersensitivity-induced chronic gastrointestinal inflammation in a non-human primate model of diet-induced obesity.

Authors - Tomris Mustafa
humanWB

MBio

The Extracellular Domain of the β2 Integrin β Subunit (CD18) Is Sufficient for Escherichia coli Hemolysin and Aggregatibacter actinomycetemcomitans Leukotoxin Cytotoxic Activity.

Authors - Laura C Ristow
mouseWB

Invest Ophthalmol Vis Sci

Lacrimal Gland Inflammation Deregulates Extracellular Matrix Remodeling and Alters Molecular Signature of Epithelial Stem/Progenitor Cells.

Authors - Takeshi Umazume
humanIF

Diabetes

Loss of IDO1 Expression From Human Pancreatic β-Cells Precedes Their Destruction During the Development of Type 1 Diabetes.

Authors - Florence Anquetil
humanIF

J Exp Med

PMID: 32181805

Authors - Xiaoyan Zhan