CD11c/Integrin Alpha X Monoclonal antibody

CD11c/Integrin Alpha X Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2F1C10

Cat no : 60258-1-Ig

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6



Tested Applications

Positive WB detected inhuman spleen tissue, THP-1 cells, HL-60 cells, U-937 cells
Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue, human breast cancer tissue, human liver cancer tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60258-1-Ig is CD11c/Integrin Alpha X Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD11c/Integrin Alpha X fusion protein Ag11350
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated molecular weight 1169 aa, 129 kDa
Observed molecular weight 145 kDa
GenBank accession numberBC038237
Gene symbol ITGAX
Gene ID (NCBI) 3687
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

Protocols

Product Specific Protocols
WB protocol for CD11c/Integrin Alpha X antibody 60258-1-IgDownload protocol
IHC protocol for CD11c/Integrin Alpha X antibody 60258-1-IgDownload protocol
IF protocol for CD11c/Integrin Alpha X antibody 60258-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

Tissue-resident FOLR2+ macrophages associate with CD8+ T cell infiltration in human breast cancer.

Authors - Rodrigo Nalio Ramos
humanIF

J Exp Med

AIM2 regulates anti-tumor immunity and is a viable therapeutic target for melanoma.

Authors - Keitaro Fukuda
humanIF

J Exp Med

PMID: 32181805

Authors - Xiaoyan Zhan
humanIF

Diabetes

Loss of IDO1 Expression From Human Pancreatic β-Cells Precedes Their Destruction During the Development of Type 1 Diabetes.

Authors - Florence Anquetil
humanIF

J Invest Dermatol

Lidocaine Ameliorates Psoriasis by Obstructing Pathogenic CGRP Signaling-Mediated Sensory Neuron-Dendritic Cell Communication.

Authors - Qianqian Yin
IF

Front Immunol

In Situ Characterization of Human Lymphoid Tissue Immune Cells by Multispectral Confocal Imaging and Quantitative Image Analysis; Implications for HIV Reservoir Characterization.

Authors - Eirini Moysi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue at 1:500. We used a Tris-EDTA pH 9.0 antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Prostate