Validation Data Gallery
|Positive WB detected in||human spleen tissue, THP-1 cells, HL-60 cells, U-937 cells|
|Positive IHC detected in||human tonsillitis tissue, human lung cancer tissue, human breast cancer tissue, human liver cancer tissue, human appendicitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:10000-1:40000|
|Immunofluorescence (IF)||IF : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 60258-1-Ig is CD11c/Integrin Alpha X Fusion Protein expressed in E. coli.
|Cited Reactivity||human, pig|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||CD11c/Integrin Alpha X fusion protein Ag11350|
|Full Name||integrin, alpha X (complement component 3 receptor 4 subunit)|
|Calculated molecular weight||1169 aa, 129 kDa|
|Observed molecular weight||145 kDa|
|GenBank accession number||BC038237|
|Gene ID (NCBI)||3687|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.
|Product Specific Protocols|
|WB protocol for CD11c/Integrin Alpha X antibody 60258-1-Ig||Download protocol|
|IHC protocol for CD11c/Integrin Alpha X antibody 60258-1-Ig||Download protocol|
|IF protocol for CD11c/Integrin Alpha X antibody 60258-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Food hypersensitivity-induced chronic gastrointestinal inflammation in a non-human primate model of diet-induced obesity.
The Extracellular Domain of the β2 Integrin β Subunit (CD18) Is Sufficient for Escherichia coli Hemolysin and Aggregatibacter actinomycetemcomitans Leukotoxin Cytotoxic Activity.
Tumor β-catenin expression is associated with immune evasion in non-small cell lung cancer with high tumor mutation burden.
Exp Cell Res
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Invest Ophthalmol Vis Sci
Lacrimal Gland Inflammation Deregulates Extracellular Matrix Remodeling and Alters Molecular Signature of Epithelial Stem/Progenitor Cells.
Case Report: Dendritic Cells and Macrophages Capture Sperm in Chronically Inflamed Human Epididymis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emma (Verified Customer) (11-29-2021)
Works well by IF on FFPE tissue at 1:500. We used a Tris-EDTA pH 9.0 antigen retrieval.