Tested Applications
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-60258 targets CD11c in IF-P applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11350 Product name: Recombinant human CD11c/Integrin alpha X protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 21-352 aa of BC038237 Sequence: NLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMA Predict reactive species |
| Full Name | integrin, alpha X (complement component 3 receptor 4 subunit) |
| Calculated Molecular Weight | 1169 aa, 129 kDa |
| GenBank Accession Number | BC038237 |
| Gene Symbol | CD11c |
| Gene ID (NCBI) | 3687 |
| ENSEMBL Gene ID | ENSG00000140678 |
| RRID | AB_2883450 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20702 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis. And this anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CD11c antibody CL594-60258 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD11c Monoclonal antibody (2F1C10), catalog number CL594-60258, has a total of 39 citations. The three retrieved citations appear in JCI Insight, Mucosal Immunology, and Basic Clinical Pharmacology & Toxicology. These studies span immunology and transplantation research—examining CD103+ dendritic cells and corneal allograft survival—and toxicology, profiling PDK4 in acute lung injury. All cited applications were immunofluorescence (IF) in mouse.
| Species | Application | Title |
|---|---|---|
JCI Insight Acquired Immunostimulatory Phenotype of Migratory CD103+ Dendritic Cells Promotes Alloimmunity Following Corneal Transplantation | ||
Mucosal Immunol Conventional type I migratory CD103+ dendritic cells are required for corneal allograft survival | ||
Basic Clin Pharmacol Toxicol Multi-Omics Profiling Reveals PDK4 as a Key Regulator of Acute Lung Injury in Glufosinate-Ammonium Poisoning. |
