CoraLite®594-conjugated CD11c Monoclonal antibody

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  IF Staining of human tonsillitis using CL594-60258

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11c/Integrin Alpha X antibody (CL594-60258, Clone: 2F1C10 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-60258

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11c/Integrin Alpha X antibody (CL594-60258, Clone: 2F1C10 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-60258

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11c/Integrin Alpha X antibody (CL594-60258, Clone: 2F1C10 ) at dilution of 1:200, CoraLite®488 CD8 antibody (CL488-66868, Clone: 1G2B10, green).

CD11c Monoclonal Antibody for IF-P

Cat No. CL594-60258

Clone No.2F1C10

Publications(2)

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

IF-P

Synonyms

2F1C10, CD11 antigen-like family member C, Integrin alpha X, Integrin alpha-X, ITGAX

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

CL594-60258 targets CD11c in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD11c fusion protein Ag11350
• Full Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Calculated Molecular Weight: 1169 aa, 129 kDa
• GenBank Accession Number: BC038237
• Gene Symbol: CD11c
• Gene ID (NCBI): 3687
• ENSEMBL Gene ID: ENSG00000140678
• RRID: AB_2883450
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20702
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis. And this anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Publications

Species	Application	Title
mouse	IF	JCI Insight; Acquired Immunostimulatory Phenotype of Migratory CD103+ Dendritic Cells Promotes Alloimmunity Following Corneal Transplantation; Authors - Tomas Blanco
mouse	IF	Mucosal Immunol; Conventional type I migratory CD103+ dendritic cells are required for corneal allograft survival; Authors - Tomas Blanco