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CoraLite® Plus 488-conjugated CD11c/Integrin Alpha X Monoclonal antibody

CD11c/Integrin Alpha X Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F1C10

Cat no : CL488-60258

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated CD11c/Integrin Alpha (CL488-60258)

    IF Staining of human tonsillitis using CL488-60258

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD11c/Integrin Alpha X antibody (CL488-60258, Clone: 2F1C10 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated CD11c/Integrin Alpha (CL488-60258)

    IF Staining of human tonsillitis using CL488-60258

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD11c/Integrin Alpha X antibody (CL488-60258, Clone: 2F1C10 ) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

CL488-60258 targets CD11c/Integrin Alpha X in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD11c/Integrin Alpha X fusion protein Ag11350
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated Molecular Weight 1169 aa, 129 kDa
GenBank Accession NumberBC038237
Gene Symbol ITGAX
Gene ID (NCBI) 3687
RRIDAB_2883128
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis. And this anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD11c/Integrin Alpha X antibody CL488-60258Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Clin Cancer Res

18F-FMISO PET Imaging Identifies Hypoxia and Immunosuppressive Tumor Microenvironments and Guides Targeted Evofosfamide Therapy in Tumors Refractory to PD-1 and CTLA-4 Inhibition.

Authors - Kirsten M Reeves
View Article
IF

Inflamm Res

Long-chain acyl-CoA synthetase 4-mediated mitochondrial fatty acid metabolism and dendritic cell antigen presentation

Authors - Yan Li
View Article