Tested Applications
| Positive WB detected in | HeLa cells, C6 cells, HEK-293 cells, HSC-T6 cells, ROS1728 cells, Jurkat cells, Raji cells, K-562 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66418-1-Ig targets IkB Alpha in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22040 Product name: Recombinant human NFKBIA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 20-217 aa of BC004983 Sequence: LKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNG Predict reactive species |
| Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC004983 |
| Gene Symbol | IkB Alpha |
| Gene ID (NCBI) | 4792 |
| RRID | AB_2881790 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P25963 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IkB Alpha antibody 66418-1-Ig | Download protocol |
| WB protocol for IkB Alpha antibody 66418-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IkB Alpha Monoclonal antibody (Cat# 66418-1-Ig) has 72 citations. These appear in journals including Nature Communications, Autophagy, Journal of Advanced Research, Journal of Experimental & Clinical Cancer Research, Advanced Science, Cell Reports, International Immunopharmacology, Journal of Ethnopharmacology, Food & Function, and Scientific Reports. Research fields span NF-κB signaling, inflammation, cancer, sepsis, neuroinflammation, liver and kidney injury, gut microbiota, and traditional Chinese medicine studies, with applications mainly in Western blot, IF, and IHC across human, mouse, rat, and pig models.
| Species | Application | Title |
|---|---|---|
Autophagy Copper aggravated synaptic damage after traumatic brain injury by downregulating BNIP3-mediated mitophagy | ||
Nat Commun Gemcitabine plus selinexor in selective advanced sarcomas: a phase I of the Spanish group for research on sarcoma study. | ||
J Adv Res Integrated multi-omics reveals the Bacillus amyloliquefaciens BA40 against Clostridium perfringens infection in weaned piglets | ||
J Exp Clin Cancer Res Superior anti-DLBCL efficacy of novel organic arsenical Z2-A-Z2 through ROS-mediated apoptosis and critical NF-κB/IκBα signaling pathway inhibition. | ||
Adv Sci (Weinh) A Computational Strategy for Identifying Self-Assembling Food-Derived Molecules for Antiparasitic Nanotherapy. | ||
Carbohydr Polym The structural discrepancy between the ability of fructan and arabinogalactan to cure acute pharyngitis in Hosta plantaginea (Lam.) Aschers flowers |
Reviews
What customers say
The single review rates the antibody 4 out of 5 stars for Western Blot on oral squamous cell carcinoma tissue. The user used a 1:1000 dilution and reported it worked well at that concentration, though they noted it performed slightly less effectively than the recommended 1:5000 dilution. Overall, the feedback is positive, with the antibody delivering reliable results in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Baraa (Verified Customer) (12-07-2025) | Worked good on the reported concentration yet not as good as the recommended 1:5000
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