IkB Alpha Monoclonal antibody

IkB Alpha Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2G6H7

Cat no : 66418-1-Ig

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA



Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, C6 cells, ROS1728 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells, HEK-293 cells, Jurkat cells, Raji cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

66418-1-Ig targets IkB Alpha in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IkB Alpha fusion protein Ag22040
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004983
Gene symbol NFKBIA
Gene ID (NCBI) 4792
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Protocols

Product Specific Protocols
WB protocol for IkB Alpha antibody 66418-1-IgDownload protocol
IHC protocol for IkB Alpha antibody 66418-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Am J Transl Res

Isoliquiritigenin attenuates LPS-induced AKI by suppression of inflammation involving NF-κB pathway.

Authors - Yun Tang
humanWB

Mol Med Rep

Downregulation of transgelin 2 promotes breast cancer metastasis by activating the reactive oxygen species/nuclear factor‑κB signaling pathway.

Authors - Liu Yang
ratWB

Food Funct

Astaxanthin attenuates oxidative stress and immune impairment in D-galactose-induced aging in rats by activating the Nrf2/Keap1 pathway and suppressing the NF-κB pathway.

Authors - Zhiqing Chen