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IkB Alpha Monoclonal antibody, PBS Only (Capture) (66418-1-PBS)

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IkB Alpha Monoclonal antibody, PBS Only (Capture)

Name: IkB Alpha Monoclonal antibody, PBS Only (Capture), 100ug
Brand: Proteintech
SKU: 66418-1-PBS-100UG

IkB Alpha Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 66418-1-PBS

Clone No. 2G6H7

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

IKBA, IκB α, NFKBIA, 2G6H7, I kappa B alpha

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllWBIHCCytometric bead arrayAffinity

WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.
WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.
WB analysis using 66418-1-Ig (same clone as 66418-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 66418-1-Ig (same clone as 66418-1-PBS)
WB result of IkB Alpha antibody (66418-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IkB Alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of C6 using 66418-1-Ig (same clone as 66418-1-PBS)
C6 cells were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of ROS1728 using 66418-1-Ig (same clone as 66418-1-PBS)
ROS1728 cells were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human prostate cancer using 66418-1-Ig (same clone as 66418-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66418-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP51027-2
Cytometric bead array standard curve of MP51027-2, NFKBIA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66418-1-PBS. Detection antibody: 60712-1-PBS. Standard:Ag0345. Range: 0.391-100 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 66418-1-Ig
Biolayer interferometry (BLl) kinetic assay of 66418-1-Ig against Human NFKBIA was performed. The affinity constant is 0.703 nM...
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)

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MSDS SDS

Product Information

66418-1-PBS targets IkB Alpha as part of a matched antibody pair:

MP51027-2: 66418-1-PBS capture and 60712-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag22040 Product name: Recombinant human NFKBIA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 20-217 aa of BC004983 Sequence: LKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNG Predict reactive species
Full Name	nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	36 kDa
GenBank Accession Number	BC004983
Gene Symbol	IkB Alpha
Gene ID (NCBI)	4792
RRID	AB_2881790
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P25963
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Publications

What published studies show

The IkB Alpha Monoclonal antibody (2G6H7), catalog number 66418-1-PBS, has a total of 70 citations. One retrieved citation appears in Neoplasia (Impact Factor 4.8), published in 2025, using WB in mouse samples. The research field involves bone cancer pain, neuronal oxidative stress, and microglial inflammation.

Species	Application	Title
mouse	WB	Neoplasia ZC3H15 suppression ameliorates bone cancer pain through inhibiting neuronal oxidative stress and microglial inflammation Authors - Li-Quan Huang View Article