Integrin Beta 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Integrin Beta 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

29

Cat no : 26918-1-AP

Synonyms

CD29, FNRB, GPIIA, Integrin beta 1, Integrin beta-1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB

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Validation Data Gallery

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  WB analysis using 26918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26918-1-AP (Integrin Beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 26918-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 26918-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 26918-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 26918-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26918-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue, HT-1080 cells, rat lung tissue
• Positive IHC detected in: human skin tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• FC: See 1 publications below

Product Information

26918-1-AP targets Integrin Beta 1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, Hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin Beta 1 fusion protein Ag25426
• Full Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC020057
• Gene Symbol: ITGB1
• Gene ID (NCBI): 3688
• RRID: AB_2880685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Regulation of local GTP availability controls RAC1 activity and cell invasion; Authors - Anna Bianchi-Smiraglia
rat	WB	Theranostics; A functionalized self-assembling peptide containing E7 and YIGSR sequences enhances neuronal differentiation of spermatogonial stem cells on aligned PCL fibers for spinal cord injury repair; Authors - Zhiyuan Wang
human	WB,IF	Mater Today Bio; Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy.; Authors - Lili Liu
rat	WB	ACS Appl Mater Interfaces; Engineered Biomimetic Nanoplatform Protects the Myocardium Against Ischemia/Reperfusion Injury by Inhibiting Pyroptosis.; Authors - Yazhong Wei
human	WB	Cell Chem Biol; Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4.; Authors - David W Wolff
mouse	WB,IHC	Biomed Pharmacother; Triptolide attenuates pulmonary fibrosis by inhibiting fibrotic extracellular matrix remodeling mediated by MMPs/LOX/integrin; Authors - Weiji Lin

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