Integrin Beta 1 Polyclonal antibody

Integrin Beta 1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 26918-1-AP

Synonyms

CD29, FNRB, GPIIA, Integrin beta 1, Integrin beta-1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB

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Validation Data Gallery

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  WB analysis of HT-1080 using 26918-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin using 26918-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 26918-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 26918-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26918-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-1080 cells
• Positive IHC detected in: human skin tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• FC: See 1 publications below

Product Information

The immunogen of 26918-1-AP is Integrin Beta 1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, Hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin Beta 1 fusion protein Ag25426
• Full Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Calculated molecular weight: 88 kDa
• Observed molecular weight: 130 kDa
• GenBank accession number: BC020057
• Gene symbol: ITGB1
• Gene ID (NCBI): 3688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	J Diabetes Res; Protective Role of Tangshen Formula on the Progression of Renal Damage in db/db Mice by TRPC6/Talin1 Pathway in Podocytes.; Authors - Qian Wang
human	WB	Cell Death Discov; FN-EDA mediates angiogenesis of hepatic fibrosis via integrin-VEGFR2 in a CD63 synergetic manner.; Authors - Xiaonan Su
rat	WB	ACS Appl Mater Interfaces; Engineered Biomimetic Nanoplatform Protects the Myocardium Against Ischemia/Reperfusion Injury by Inhibiting Pyroptosis.; Authors - Yazhong Wei
human	WB,IF	Mater Today Bio; Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy.; Authors - Lili Liu
	WB	Theriogenology; Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.; Authors - Xue Wang
human	IF	Cancer Cell Int; Silencing Formin-like 2 inhibits growth and metastasis of gastric cancer cells through suppressing internalization of integrins.; Authors - Banghua Zhong

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