Integrin Beta 1/CD29 Monoclonal antibody

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• Integrin Beta 1/CD29 Monoclonal antibody (66315-1-Ig)

Integrin Beta 1/CD29 Monoclonal Antibody for WB, ELISA

Cat No. 66315-1-Ig Copied!

Clone No. 1A7C9

Publications (3)

Reviews (1)

Download datasheet

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

ITGB1, 1A7C9, CD29, FNRB, Glycoprotein IIa

• 

  WB analysis of HT-1080 using 66315-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HUVEC using 66315-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HT-1080 using 66315-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HT-1080 cells, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66315-1-Ig targets Integrin Beta 1/CD29 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24476

  Product name: Recombinant human Integrin beta-1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 30-359 aa of BC020057

  Sequence: ANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGT

• Full Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC020057
• Gene Symbol: Integrin beta 1
• Gene ID (NCBI): 3688
• ENSEMBL Gene ID: ENSG00000150093
• RRID: AB_2881696
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P05556
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Publications

What published studies show

The Integrin Beta 1/CD29 Monoclonal antibody (1A7C9), catalog number 66315-1-Ig, has 3 citations. They appear in Structure, Frontiers in Medicine (Lausanne), and bioRxiv. The cited research spans integrin signaling and phosphorylation regulation, mechanobiology of nucleus pulposus cells involving the TRPV4/CRT/FA complex, and Wnt/APC-mediated cholesterol metabolism via SREBP2. All three studies used the antibody for Western blot in human or rat samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Structure; Phosphorylation of RIAM by Src Promotes Integrin Activation by Unmasking the PH Domain of RIAM.; Authors - Eun-Ah Cho
rat	WB	Front Med (Lausanne); Low hydrostatic pressure promotes functional homeostasis of nucleus pulposus cells through the TRPV4/CRT/FA complex axis; Authors - Junxian Hu
human	WB	bioRxiv; The Wnt/APC destruction complex targets SREBP2 in a β-catenin-independent pathway to control cholesterol metabolism.; Authors - Ahmed Rattani

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

The single review for this antibody rates it 4 out of 5 stars. It was used for immunohistochemistry on zebrafish retina and brain tissue at a 1:100 dilution, with Tris HCl (0.1M, pH 9) antigen retrieval. The reviewer included an image of the staining result. Overall, the feedback suggests the antibody performs well for IHC applications in zebrafish tissues.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Ryan (Verified Customer) (01-24-2018)

Antigen retrieval - Tris HCl 0.1M ph 9

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish Retina/Brain

Integrin Beta 1/CD29 Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina/Brain (Cat no:66315-1-Ig)