Integrin Beta 1/CD29 Monoclonal antibody, PBS Only

• 

  WB analysis of HT-1080 using 66315-1-Ig (same clone as 66315-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66315-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HUVEC using 66315-1-Ig (same clone as 66315-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66315-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-1080 using 66315-1-Ig (same clone as 66315-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66315-1-PBS in a different storage buffer formulation.

Integrin Beta 1/CD29 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66315-1-PBS

Clone No.1A7C9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

ITGB1, 1A7C9, CD29, FNRB, Glycoprotein IIa

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Product Information

66315-1-PBS targets Integrin Beta 1/CD29 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24476

  Product name: Recombinant human Integrin beta-1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 30-359 aa of BC020057

  Sequence: ANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGT

• Full Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC020057
• Gene Symbol: Integrin beta 1
• Gene ID (NCBI): 3688
• ENSEMBL Gene ID: ENSG00000150093
• RRID: AB_2881696
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P05556
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.