Anti-Human JAM-A/CD321 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using 98328-3-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human JAM-A/CD321 Rabbit RecAb (98328-3-RR, Clone: 242290B3) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98328-3-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human JAM-A/CD321 Rabbit RecAb (98328-3-RR, Clone:242290B3) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

JAM-A/CD321 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98328-3-RR

Clone No.242290B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

F11R, JAM-A/Junctional Adhesion Molecule A, 242290B3, CD321, F11 receptor

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98328-3-RR targets JAM-A/CD321 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: JAM-A/CD321 fusion protein Eg1372
• Full Name: F11 receptor
• Calculated Molecular Weight: 33kDa
• GenBank Accession Number: NM_016946.6
• Gene Symbol: F11R
• Gene ID (NCBI): 50848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y624-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Junctional adhesion molecule A (JAM-A), also known as CD321 or F11 receptor (F11R), was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and a ligand for the integrin LFA1.