JNK Monoclonal antibody

• Featured Product
• KD/KO Validated

JNK Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

CloneNo.

1A12E1

Cat no : 66210-1-Ig

Synonyms

c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1

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Validation Data Gallery

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  WB analysis using 66210-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66210-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66210-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66210-1-Ig

  Immunofluorescent analysis of HeLa cells using 66210-1-Ig (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells, C6 cells, RAW 264.7 cells, Jurkat cells, Ethacrynic acid treated HeLa cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human lung cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:20000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below

Product Information

66210-1-Ig targets JNK in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: JNK fusion protein Ag21426
• Full Name: mitogen-activated protein kinase 8
• Calculated molecular weight: 48 kDa
• Observed molecular weight: 44-48 kDa, 50-55 kDa
• GenBank accession number: BC130572
• Gene symbol: MAPK8
• Gene ID (NCBI): 5599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 – MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

• JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

Publications

Species	Application	Title
human	WB	BMC Cancer; MicroRNA-148b regulates tumor growth of non-small cell lung cancer through targeting MAPK/JNK pathway.; Authors - Lin Lu
mouse	WB	Free Radic Biol Med; Cathelicidin-WA attenuates LPS-induced inflammation and redox imbalance through activation of AMPK signaling.; Authors - Weiche Wu
human	WB	Med Oncol; Association and clinicopathologic significance of p38MAPK-ERK-JNK-CDC25C with polyploid giant cancer cell formation.; Authors - Kai Liu
mouse	WB	Exp Cell Res; Involvement of JNK signaling in IL4-induced M2 macrophage polarization.; Authors - Jin Hao ; KD Validated
rat	WB	Life Sci; Chlorogenic acid attenuates cyclophosphamide-induced rat interstitial cystitis.; Authors - Jing Luo
mouse	WB	Life Sci; Inhibiting the Piezo1 channel protects microglia from acute hyperglycaemia damage through the JNK1 and mTOR signalling pathways.; Authors - Hailin Liu

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