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JNK Antibody 3 Publications

Mouse Monoclonal| Catalog number: 66210-1-Ig |CloneNo.: 1A12E1

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Con: 120 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

JNK Antibody WB HeLa cells 66210-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

JNK Antibody IHC human lung cancer tissue 66210-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

JNK Antibody IHC human lung cancer tissue 66210-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

JNK Antibody IF HeLa cells 66210-1-Ig

Immunofluorescent analysis of HeLa cells using 66210-1-Ig(JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, SH-SY5Y cells, C6 cells, RAW 264.7 cells, Jurkat cells, Ethacrynic acid treated HeLa cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected in:
human lung cancer tissue, human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:3000-1:20000
IHC : 1:500-1:2000
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
JNK fusion protein Ag21426
Full name:
mitogen-activated protein kinase 8
Calculated molecular weight:
48 kDa
Observed molecular weight:
40-48 kDa, 55 kDa
GenBank accession number:
BC130572
Gene ID (NCBI):
5599
Gene symbol
MAPK8
Synonyms
c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1

Background

Function

JNK1, also known as mitogen-activated protein kinase 8 – MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  


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