- Featured Product
- KD/KO Validated
CoraLite®488-conjugated JNK Monoclonal antibody
JNK Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
CoraLite®488 Fluorescent Dye
Cat no : CL488-66210
|Positive IF detected in||HeLa cells|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
CL488-66210 targets JNK in IF applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG1|
|Immunogen||JNK fusion protein Ag21426|
|Full Name||mitogen-activated protein kinase 8|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||44-48 kDa, 50-55 kDa|
|GenBank accession number||BC130572|
|Gene ID (NCBI)||5599|
|Conjugate||CoraLite®488 Fluorescent Dye|
|Excitation/Emission maxima wavelengths||488 nm/515 nm|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.|
JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.
JNK1 is ubiquitously expressed.
Involvement in disease
JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.
There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.
JNK1 is phosphorylated by MAPK kinases (MAPKK).
JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.