JNK Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

JNK Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A12E1

Cat no : 66210-1-PBS

Synonyms

c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1

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Validation Data Gallery

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  WB analysis using 66210-1-Ig (same clone as 66210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  WB analysis using 66210-1-Ig (same clone as 66210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66210-1-Ig (same clone as 66210-1-PBS)

  Ethacrynic acid treated HeLa cells were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66210-1-Ig (same clone as 66210-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 66210-1-Ig (same clone as 66210-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66210-1-Ig (same clone as 66210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66210-1-Ig (same clone as 66210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66210-1-Ig (same clone as 66210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66210-1-Ig (same clone as 66210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66210-1-Ig (same clone as 66210-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human JNK (66210-1-Ig, Clone:1A12E1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66210-1-Ig (same clone as 66210-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human JNK (66210-1-Ig, Clone:1A12E1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66210-1-PBS targets JNK in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: JNK fusion protein Ag21426
• Full Name: mitogen-activated protein kinase 8
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 44-48 kDa, 50-55 kDa
• GenBank Accession Number: BC130572
• Gene Symbol: MAPK8
• Gene ID (NCBI): 5599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

• JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.