KCNMA1 Polyclonal antibody

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  WB analysis using 22158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22158-1-AP (KCNMA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 22158-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KCNMA1 antibody (22158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 22158-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KCNMA1 antibody (22158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KCNMA1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 22158-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

BK channel, BKCA alpha, BKTM, hSlo, K(VCA)alpha

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Tested Applications

• Positive WB detected in: mouse brain membrane protein, rat brain membrane protein
• Positive IHC detected in: mouse cerebellum tissue, mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22158-1-AP targets KCNMA1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17802

  Product name: Recombinant human KCNMA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 829-1178 aa of BC137115

  Sequence: KREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRQNRPKSRESRDKQKYVQEERL

• Full Name: potassium large conductance calcium-activated channel, subfamily M, alpha member 1
• Calculated Molecular Weight: 1236 aa, 138 kDa
• Observed Molecular Weight: ~130 kDa
• GenBank Accession Number: BC137115
• Gene Symbol: KCNMA1
• Gene ID (NCBI): 3778
• RRID: AB_3669389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12791
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calcium-activated potassium channel subunit alpha-1 also known as large conductance calcium-activated potassium channel, subfamily M, alpha member 1 (KCa1.1), or BK channel alpha subunit, is a voltage-gated potassium channel encoded by the KCNMA1 gene and characterized by their large conductance of potassium ions (K+) through cell membranes. KCNMA1 is widely distributed in human cell membranes, especially in muscle cells and neurons. They regulate the excitability of cells through sensitivity to calcium ions (Ca²⁺) and voltage, thereby affecting key physiological processes such as muscle contraction and nerve signal transduction.