KCNQ3 Polyclonal antibody

KCNQ3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 19966-1-AP

Synonyms

Potassium voltage-gated channel subfamily KQT member 3, KV7.3, KQT-like 3, KQT like 3, EBN2

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Validation Data Gallery

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  WB analysis of pig heart using 19966-1-AP

  pig heart tissue were subjected to SDS PAGE followed by western blot with 19966-1-AP (KCNQ3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19966-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19966-1-AP (KCNQ3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19966-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19966-1-AP (KCNQ3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: pig heart tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

19966-1-AP targets KCNQ3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: potassium voltage-gated channel, KQT-like subfamily, member 3
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: NM_004519
• Gene Symbol: KCNQ3
• Gene ID (NCBI): 3786
• RRID: AB_2878632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCNQ3, also named as BFNC2, EBN2 and KV7.3, belongs to the potassium channel family and KQT subfamily. KCNQ3 is probably important in the regulation of neuronal excitability. Associates with KCNQ2 or KCNQ5, KCNQ3 forms a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. Defects in KCNQ3 are the cause of benign neonatal epilepsy type 2 (EBN2). The antibody recognizes the C-term of KCNQ3.

Publications

Species	Application	Title
mouse	WB	Hippocampus; Impacted spike frequency adaptation associated with reduction of KCNQ2/3 exacerbates seizure activity in temporal lobe epilepsy; Authors - Shicheng Jiang
	WB	Genes (Basel); Differential Dorsolateral Prefrontal Cortex Proteomic Profiles of Suicide Victims with Mood Disorders.; Authors - Alejandra Cabello-Arreola