Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55409-1-AP targets KHSRP in WB, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | KH-type splicing regulatory protein |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | NM_003685 |
| Gene Symbol | KHSRP |
| Gene ID (NCBI) | 8570 |
| RRID | AB_11182170 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92945 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KHSRP antibody 55409-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
KHSRP Polyclonal antibody (Cat# 55409-1-AP) has 9 total citations published in Nucleic Acids Research, Advanced Science, Cell Reports, Atherosclerosis, International Immunopharmacology, American Journal of Physiology–Cell Physiology, iScience, and Biochimica et Biophysica Acta. Associated research fields include mRNA stability and alternative splicing, cancer (breast, colorectal, lung), embryonic development, lipid metabolism, intratumoral immunosuppression, angiogenesis, mitochondrial dysfunction, ciliopathy, and viral infection.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities. | ||
Adv Sci (Weinh) Targeting lncRNA DDIT4-AS1 Sensitizes Triple Negative Breast Cancer to Chemotherapy via Suppressing of Autophagy | ||
Cell Rep Identification of cis-HOX-HOXC10 axis as a therapeutic target for colorectal tumor-initiating cells without APC mutations.
| ||
Atherosclerosis microRNA-185 modulates low density lipoprotein receptor expression as a key posttranscriptional regulator. | ||
Int Immunopharmacol Angiotensin II contributes to intratumoral immunosuppressionvia induction of PD-L1 expression in non-small cell lung carcinoma.
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