KHSRP Polyclonal antibody

• Featured Product
• KD/KO Validated

KHSRP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, RIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 55409-1-AP

Synonyms

FBP2, FUBP2, FUSE binding protein 2, KHSRP, KSRP, p75

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Validation Data Gallery

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  WB analysis using 55409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55409-1-AP (KHSRP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55409-1-AP

  WB result of KHSRP antibody (55409-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-KHSRP transfected HeLa cells.

• 

  WB analysis using 55409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55409-1-AP (KHSRP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Product Information

55409-1-AP targets KHSRP in WB, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: KH-type splicing regulatory protein
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: NM_003685
• Gene Symbol: KHSRP
• Gene ID (NCBI): 8570
• RRID: AB_11182170
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities.; Authors - Xiaomei He
human	WB,IF	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	Atherosclerosis; microRNA-185 modulates low density lipoprotein receptor expression as a key posttranscriptional regulator.; Authors - Huajun Jiang
human	WB	Int Immunopharmacol; Angiotensin II contributes to intratumoral immunosuppressionvia induction of PD-L1 expression in non-small cell lung carcinoma.; Authors - Kaiyong Yang ; KD Validated
human	WB,RIP	Cell Rep; Identification of cis-HOX-HOXC10 axis as a therapeutic target for colorectal tumor-initiating cells without APC mutations.; Authors - Zhenzhen Chen ; KO Validated
human	WB, IF	Biochim Biophys Acta; Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection.; Authors - Fan Chai