KIAA0319L Polyclonal antibody

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  WB analysis using 21016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21016-1-AP (KIAA0319L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21016-1-AP (KIAA0319L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21016-1-AP (KIAA0319L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21016-1-AP (KIAA0319L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

KIAA0319L Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21016-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

PKD1 like, KIAA1837, KIAA0319 like, Dyslexia-associated protein KIAA0319-like protein, Adeno-associated virus receptor

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Tested Applications

• Positive WB detected in: HeLa cells, HuH-7 cells, MCF-7 cells, NIH/3T3 cells, Neuro-2a cells, C6 cells
• Positive IHC detected in: rat brain tissue, human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 2 publications below

Product Information

21016-1-AP targets KIAA0319L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15263

  Product name: Recombinant human KIAA0319L protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 233-582 aa of BC031672

  Sequence: ATAQVTVIVQPENNKPPQADAGPDKELTLPVDSTTLDGSKSSDDQKIISYLWEKTQGPDGVQLENANSSVATVTGLQVGTYVFTLTVKDERNLQSQSSVNVIVKEEINKPPIAKITGNVVITLPTSTAELDGSKSSDDKGIVSYLWTRDEGSPAAGEVLNHSDHHPILFLSNLVEGTYTFHLKVTDAKGESDTDRTTVEVKPDPRKNNLVEIILDINVSQLTERLKGMFIRQIGVLLGVLDSDIIVQKIQPYTEQSTKMVFFVQNEPPHQIFKGHEVAAMLKSELRKQKADFLIFRALEVNTVTCQLNCSDHGHCDSFTKRCICDPFWMENFIKVQLRDGDSNCEWSVLY

• Full Name: KIAA0319-like
• Calculated Molecular Weight: 1049 aa, 116 kDa
• Observed Molecular Weight: ~150 kDa
• GenBank Accession Number: BC031672
• Gene Symbol: KIAA0319L
• Gene ID (NCBI): 79932
• RRID: AB_10733523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IZA0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA0319L (also known as AAVR) is a type-I transmembrane protein that acts as a receptor for adeno-associated virus (AAV) and is involved in adeno-associated virus infection through endocytosis system (PMID: 26814968). It has been shown that KIAA0319L is highly-glycosylated and can localise to the plasma membrane, endosomes, Golgi and trans-Golgi network (TGN) (PMID: 29991750). KIAA0319L gene has been associated with susceptibility to developmental dyslexia or reading disabilities (PMID: 19085271). The predicted molecular mass of KIAA0319L is 116 kDa, but western blot detected protein at 150 kDa in lysate from HeLa cells, likely due to glycosidic linkages (PMID: 28679762; 30962536).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Methods; Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression; Authors - James Zengel
human	WB	Sci Adv; Enhancement of liver-directed transgene expression at initial and repeat doses of AAV vectors admixed with ImmTOR nanoparticles.; Authors - Petr O Ilyinskii ; KD Validated
mouse	WB	Sci Rep; AU040320 deficiency leads to disruption of acrosome biogenesis and infertility in homozygous mutant mice.; Authors - Luiz G Guidi
human,mouse	IF	Gene Ther; Serotype survey of AAV gene delivery via subconjunctival injection in mice.; Authors - Liujiang Song
human	WB	Sci Rep; Correction of LAMP3-associated salivary gland hypofunction by aquaporin gene therapy; Authors - Hiroyuki Nakamura
human	WB	bioRxiv; Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors; Authors - Xiujuan Zhang