KIAA0319L Polyclonal antibody

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• KIAA0319L Polyclonal antibody (21016-1-AP)

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• KD/KO Validated

KIAA0319L Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21016-1-AP Copied!

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

AAVR, Adeno-associated virus receptor, Dyslexia-associated protein KIAA0319-like protein, KIAA0319 like, KIAA1837

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  WB analysis using 21016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21016-1-AP (KIAA0319L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 21016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21016-1-AP (KIAA0319L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21016-1-AP (KIAA0319L antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21016-1-AP (KIAA0319L antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HuH-7 cells, MCF-7 cells, NIH/3T3 cells, Neuro-2a cells, C6 cells
• Positive IHC detected in: mouse brain tissue, human brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21016-1-AP targets KIAA0319L in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15263

  Product name: Recombinant human KIAA0319L protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 233-582 aa of BC031672

  Sequence: ATAQVTVIVQPENNKPPQADAGPDKELTLPVDSTTLDGSKSSDDQKIISYLWEKTQGPDGVQLENANSSVATVTGLQVGTYVFTLTVKDERNLQSQSSVNVIVKEEINKPPIAKITGNVVITLPTSTAELDGSKSSDDKGIVSYLWTRDEGSPAAGEVLNHSDHHPILFLSNLVEGTYTFHLKVTDAKGESDTDRTTVEVKPDPRKNNLVEIILDINVSQLTERLKGMFIRQIGVLLGVLDSDIIVQKIQPYTEQSTKMVFFVQNEPPHQIFKGHEVAAMLKSELRKQKADFLIFRALEVNTVTCQLNCSDHGHCDSFTKRCICDPFWMENFIKVQLRDGDSNCEWSVLY

• Full Name: KIAA0319-like
• Calculated Molecular Weight: 1049 aa, 116 kDa
• Observed Molecular Weight: ~150 kDa
• GenBank Accession Number: BC031672
• Gene Symbol: KIAA0319L
• Gene ID (NCBI): 79932
• RRID: AB_10733523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IZA0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA0319L (also known as AAVR) is a type-I transmembrane protein that acts as a receptor for adeno-associated virus (AAV) and is involved in adeno-associated virus infection through endocytosis system (PMID: 26814968). It has been shown that KIAA0319L is highly-glycosylated and can localise to the plasma membrane, endosomes, Golgi and trans-Golgi network (TGN) (PMID: 29991750). KIAA0319L gene has been associated with susceptibility to developmental dyslexia or reading disabilities (PMID: 19085271). The predicted molecular mass of KIAA0319L is 116 kDa, but western blot detected protein at 150 kDa in lysate from HeLa cells, likely due to glycosidic linkages (PMID: 28679762; 30962536).

Publications

What published studies show

The KIAA0319L (AAVR) Polyclonal Antibody (Cat# 21016-1-AP) has 13 citations published in journals including Nature Biotechnology, Nature Methods, Science Advances, Biomaterials, Gene Therapy, mBio, Scientific Reports, Structure, Analytical Bioanalytical Chemistry, and Molecular Therapy Advances. Research fields span AAV gene therapy and transduction mechanisms, capsid engineering, glycoproteomics, endosomal trafficking, retinal and liver-directed gene delivery, nanocarrier imaging, and acrosome biogenesis.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Nat Biotechnol; Nanocarrier imaging at single-cell resolution across entire mouse bodies with deep learning; Authors - Jie Luo
mouse	WB	Nat Methods; Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression; Authors - James Zengel
human	WB	Sci Adv; Enhancement of liver-directed transgene expression at initial and repeat doses of AAV vectors admixed with ImmTOR nanoparticles.; Authors - Petr O Ilyinskii ; KD Validated
human, mouse	WB	Biomaterials; Precision glycoengineered AAV capsids enhance hepatocyte targeting and attenuated immune activation for liver-directed gene therapy.; Authors - Jinhuan Luo
mouse,human	IF	Gene Ther; Serotype survey of AAV gene delivery via subconjunctival injection in mice.; Authors - Liujiang Song
human	WB	mBio; Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors; Authors - Xiujuan Zhang ; KO Validated