|Positive WB detected in||mouse brain tissue, human cerebellum tissue, mouse cerebellum tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13930-1-AP targets KIF3A in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KIF3A fusion protein Ag4954|
|Full Name||kinesin family member 3A|
|Calculated molecular weight||80 kDa|
|Observed molecular weight||80 kDa|
|GenBank accession number||BC045542|
|Gene ID (NCBI)||11127|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
KIF3A is a subunit of Kinesin II that is essential for intraflagellar transport (IFT). It functions as the anterograde motor to deliver ciliary components to the tip where the axoneme is assembled. Disruption of KIF3A has been reported to block mammalian ciliogenesis. Recently KIF3A has been found to localize to the mother centriole and is required for centrioles organization. This antibody specifically recognizes the endogenous KIF3A protein in mouse embryonic fibroblasts (MEFs) but not in KIF3A-/- lysate. (23386061)
Proc Natl Acad Sci U S A
EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.
Front Cell Dev Biol
Roles for IFT172 and Primary Cilia in Cell Migration, Cell Division, and Neocortex Development.
The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium.
Mol Cell Biochem
Type I collagen-induced YAP nuclear expression promotes primary cilia growth and contributes to cell migration in confluent mouse embryo fibroblast 3T3-L1 cells.
DAB2IP modulates primary cilia formation associated with renal tumorigenesis.
Arborization of dendrites by developing neocortical neurons is dependent on primary cilia and type 3 adenylyl cyclase.