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  • KD/KO Validated

KIF3A Polyclonal antibody

KIF3A Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13930-1-AP

Synonyms

KIF3, KIF3A, kinesin family member 3A, Kinesin like protein KIF3A



Tested Applications

Positive WB detected inmouse brain tissue, human cerebellum tissue, mouse cerebellum tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13930-1-AP targets KIF3A in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF3A fusion protein Ag4954
Full Name kinesin family member 3A
Calculated molecular weight 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC045542
Gene symbol KIF3A
Gene ID (NCBI) 11127
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIF3A is a subunit of Kinesin II that is essential for intraflagellar transport (IFT). It functions as the anterograde motor to deliver ciliary components to the tip where the axoneme is assembled. Disruption of KIF3A has been reported to block mammalian ciliogenesis. Recently KIF3A has been found to localize to the mother centriole and is required for centrioles organization. This antibody specifically recognizes the endogenous KIF3A protein in mouse embryonic fibroblasts (MEFs) but not in KIF3A-/- lysate. (23386061)

Protocols

Product Specific Protocols
WB protocol for KIF3A antibody 13930-1-APDownload protocol
IHC protocol for KIF3A antibody 13930-1-APDownload protocol
IP protocol for KIF3A antibody 13930-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.

Authors - Vincent J Guen
  • KO Validated
mouseWB

Front Cell Dev Biol

Roles for IFT172 and Primary Cilia in Cell Migration, Cell Division, and Neocortex Development.

Authors - Michal Pruski
mouseIF

Cell Rep

The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium.

Authors - Asma Boukhalfa
mouseWB

Mol Cell Biochem

Type I collagen-induced YAP nuclear expression promotes primary cilia growth and contributes to cell migration in confluent mouse embryo fibroblast 3T3-L1 cells.

Authors - Qian Xu
mouse,humanWB,IF

Neoplasia

DAB2IP modulates primary cilia formation associated with renal tumorigenesis.

Authors - Chun-Jung Lin
  • KD Validated
mouseWB

J Neurosci

Arborization of dendrites by developing neocortical neurons is dependent on primary cilia and type 3 adenylyl cyclase.

Authors - Guadiana Sarah M SM