Tested Applications
| Positive WB detected in | HEK-293 cells, human cerebellum tissue, mouse cerebellum tissue, SH-SY5Y cells, SK-N-SH cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13930-1-AP targets KIF3A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4954 Product name: Recombinant human KIF3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC045542 Sequence: MPINKSEKPESCDNVKVVVRCRPLNEREKSMCYKQAVSVDEMRGTITVHKTDSSNEPPKTFTFDTVFGPESKQLDVYNLTARPIIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAIPELRGIIPNSFAHIFGHIAKAEGDTRFLVRVSYLGIYNEEVRDLLGKDQTQRLEVIERPDVGVYIKDLSAYVVNNADDMDRIMTLGHKNRSVGATNMNEHSSRSHAIFTITIECSEKGIDGNMHVRMGKLHLVDLAGSERQAKTGATGQRLKEATKINLSLSTLGNVISALVDGKSTHVPYRNSKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKARINEDP Predict reactive species |
| Full Name | kinesin family member 3A |
| Calculated Molecular Weight | 80 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC045542 |
| Gene Symbol | KIF3A |
| Gene ID (NCBI) | 11127 |
| RRID | AB_2132063 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y496 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KIF3A is a subunit of Kinesin II that is essential for intraflagellar transport (IFT). It functions as the anterograde motor to deliver ciliary components to the tip where the axoneme is assembled. Disruption of KIF3A has been reported to block mammalian ciliogenesis. Recently KIF3A has been found to localize to the mother centriole and is required for centrioles organization. This antibody specifically recognizes the endogenous KIF3A protein in mouse embryonic fibroblasts (MEFs) but not in KIF3A-/- lysate. (23386061)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KIF3A antibody 13930-1-AP | Download protocol |
| IHC protocol for KIF3A antibody 13930-1-AP | Download protocol |
| IP protocol for KIF3A antibody 13930-1-AP | Download protocol |
| WB protocol for KIF3A antibody 13930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KIF3A Polyclonal antibody (Cat# 13930-1-AP) has 26 citations across journals including Nature Communications, Nature Aging, Science Advances, Cell Death & Disease, PNAS, EMBO Journal, Cell Reports, and Aging Cell. Research fields span primary cilia biology, ciliogenesis, EMT, cancer (breast, gastric, renal, pancreatic), autophagy, neurobiology, cell migration, senescence, sperm flagellum formation, and atherosclerosis.
| Species | Application | Title |
|---|---|---|
Nat Aging A primary cilia-autophagy axis in hippocampal neurons is essential to maintain cognitive resilience
| ||
Nat Commun Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. | ||
Nat Commun Lipid droplets sequester palmitic acid to disrupt endothelial ciliation and exacerbate atherosclerosis in male mice | ||
Sci Adv An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis. | ||
Cell Death Dis Inhibition of primary cilia-hedgehog signaling axis triggers autophagic cell death and suppresses malignant progression of VHL wild-type ccRCC |
Reviews
What customers say
The single review reports successful use of this antibody for Western Blot on PBMCs at a 1:1000 dilution. The user detected KIF3A with a specific band at approximately 80 kDa, consistent with the expected molecular weight. The reviewer rated the product 5 out of 5 stars, indicating strong satisfaction with its specificity and performance in detecting the target protein.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Bárbara (Verified Customer) (09-02-2025) | Western blot analyses have reported that KIF3A can be detected in PBMCs, with a specific band at approximately 80 kDa when we use a 1:1000 antibody dilution.
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