Mouse IgG2a isotype control Monoclonal antibody

Mouse IgG2a isotype control Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Megathura crenulata

Applications

WB, IP, FC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

11A1B2

Reviews (1)

Cat no : 66360-2-Ig

Synonyms

Mouse IgG2a, Mouse IgG2a isotype control

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• Validation Data Gallery
• Proteintech vs. Other Vendors

• 

  WB analysis using 66360-2-Ig

  Various lysates (50 μg/lane) were subjected to SDS PAGE followed by western blot with 66360-2-Ig (Mouse IgG2a isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 66360-2-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Mouse IgG2a isotype control (66360-2-Ig, Clone: 11A1B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Cytochrome c Mouse McAb (66264-1-Ig, Clone: 2D8D11) (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected in
Positive FC detected in

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IP: See 1 publications below
• FC: See 1 publications below

Product Information

66360-2-Ig targets Mouse IgG2a isotype control in WB, IP, FC, ELISA applications and shows reactivity with Megathura crenulata samples.

• Tested Reactivity: Megathura crenulata
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: Mouse IgG2a isotype control
• RRID: AB_2881739
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Publications

Species	Application	Title
	FC	Biosens Bioelectron; Spherical nucleic acids-based cascade signal amplification for highly sensitive detection of exosomes.; Authors - Lei Wang
	IP	Nature; CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1; Authors - Milan Hluchý

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Niklas (Verified Customer) (07-31-2023)

better staining control than secondary only control

• Applications: Immunofluorescence, Flow Cytometry
• Primary Antibody Dilution: 1:200-1:400
• Cell Tissue Type: mouse and human heart