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pan Ras Antibody

pan Ras Monoclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4H4G7

Cat no : 60309-1-Ig

Synonyms

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2B speicifc, Krasa, Krasb, NS3, pan Ras, RASK2



Tested Applications

Positive WB detected infetal human brain tissue, HEK-293 cells, Neuro-2a cells, ROS1728 cells, human brain tissue, rat brain tissue, mouse brain tissue, pig brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60309-1-Ig targets pan Ras in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen pan Ras fusion protein Ag2700
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 188aa,21 kDa
Observed molecular weight 21 kDa
GenBank accession number BC013572
Gene symbol KRAS
Gene ID (NCBI) 3845
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.

Protocols

Product Specific Protocols
WB protocol for pan Ras antibody 60309-1-IgDownload protocol
IHC protocol for pan Ras antibody 60309-1-IgDownload protocol
IP protocol for pan Ras antibody 60309-1-IgDownload protocol
FC protocol for pan Ras antibody 60309-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Pharmacother

Modulation of YrdC promotes hepatocellular carcinoma progression via MEK/ERK signaling pathway.

Authors - Shiqiong Huang
humanWB,IHC

Heliyon

FOXP1 forkhead transcription factor is associated with the pathogenesis of endometrial cancer.

Authors - Makito Mizunuma
humanWB

Oncol Rep

EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells.

Authors - Chuan Lin
humanIHC

Int J Mol Med

Overexpression of wild-type p21Ras plays a prominent role in colorectal cancer.

Authors - Shuang Bai
humanIHC

Exp Mol Med

Destabilization of β-catenin and RAS by targeting the Wnt/β-catenin pathway as a potential treatment for triple-negative breast cancer.

Authors - Won-Ji Ryu
humanWB

Cancer Res

KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis.

Authors - Peng Yuan
  • KD Validated