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pan Ras Antibody 5 Publications

Mouse Monoclonal| Catalog number: 60309-1-Ig |CloneNo.: 4H4G7

Featured Product KD/KO validated

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Con: 75 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

pan Ras Antibody WB fetal human brain tissue 60309-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig( pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours

pan Ras Antibody IP mouse brain tissue 60309-1-Ig

IP result of anti-pan Ras(IP:60309-1-Ig, 5ug; Detection:60309-1-Ig 1:2000) with mouse brain tissue lysate 4000 ug

pan Ras Antibody IHC human breast cancer tissue 60309-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig( pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

pan Ras Antibody IHC human breast cancer tissue 60309-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig( pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer(Ph9).

pan Ras Antibody FC HeLa cells 60309-1-Ig

1X10^6 HeLa cells were stained with 0.2ug pan Ras antibody (60309-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat, pig
Positive WB detected in:
fetal human brain tissue, HEK-293 cells, Neuro-2a cells, ROS1728 cells, human brain tissue, rat brain tissue, mouse brain tissue, pig brain tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human breast cancer tissue, human colon cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:20000-1:50000
IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
pan Ras fusion protein Ag2700
Full name:
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight:
188aa,21 kDa
Observed molecular weight:
21 kDa
GenBank accession number:
BC013572
Gene ID (NCBI):
3845
Gene symbol
KRAS
Synonyms
C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2B speicifc, Krasa, Krasb, NS3, pan Ras, RASK2

Background

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.


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