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pan Ras Monoclonal antibody
pan Ras Monoclonal Antibody for FC (Intra), IHC, IP, WB, ELISA
Cat no : 60309-1-Ig
Validation Data Gallery
|Positive WB detected in||fetal human brain tissue, HEK-293 cells, Neuro-2a cells, ROS1728 cells, human brain tissue, rat brain tissue, mouse brain tissue, pig brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:20000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
60309-1-Ig targets pan Ras in WB, IP, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||pan Ras fusion protein Ag2700|
|Full Name||v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog|
|Calculated molecular weight||188 aa, 21 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC013572|
|Gene ID (NCBI)||3845|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.
|Product Specific Protocols|
|WB protocol for pan Ras antibody 60309-1-Ig||Download protocol|
|IHC protocol for pan Ras antibody 60309-1-Ig||Download protocol|
|IP protocol for pan Ras antibody 60309-1-Ig||Download protocol|
|FC protocol for pan Ras antibody 60309-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells.
KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis.
Exp Mol Med
Destabilization of β-catenin and RAS by targeting the Wnt/β-catenin pathway as a potential treatment for triple-negative breast cancer.
J Cell Mol Med
DEPDC1 up-regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells.
Hyaluronic Acid-Modified Nanoparticles Self-Assembled from Linoleic Acid-Conjugated Chitosan for the Codelivery of miR34a and Doxorubicin in Resistant Breast Cancer
The immunoreactivity of the anti-p21Ras single-chain fragment variant KGH-R1 and its predicted binding sites to p21Ras.