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- KD/KO Validated
pan Ras Monoclonal antibody
pan Ras Monoclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig and More (1)
Applications
WB, IP, IHC, FC, ELISA
Conjugate
Unconjugated
CloneNo.
4H4G7
Cat no : 60309-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | fetal human brain tissue, HEK-293 cells, Neuro-2a cells, ROS1728 cells, human brain tissue, rat brain tissue, mouse brain tissue, pig brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 6 publications below |
IHC | See 3 publications below |
Product Information
60309-1-Ig targets pan Ras in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, swine |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | pan Ras fusion protein Ag2700 |
Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
Calculated molecular weight | 188 aa, 21 kDa |
Observed molecular weight | 21 kDa |
GenBank accession number | BC013572 |
Gene symbol | KRAS |
Gene ID (NCBI) | 3845 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for pan Ras antibody 60309-1-Ig | Download protocol |
IHC protocol for pan Ras antibody 60309-1-Ig | Download protocol |
IP protocol for pan Ras antibody 60309-1-Ig | Download protocol |
FC protocol for pan Ras antibody 60309-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomed Pharmacother Modulation of YrdC promotes hepatocellular carcinoma progression via MEK/ERK signaling pathway. | ||
Heliyon FOXP1 forkhead transcription factor is associated with the pathogenesis of endometrial cancer. | ||
Oncol Rep EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells. | ||
Int J Mol Med Overexpression of wild-type p21Ras plays a prominent role in colorectal cancer. | ||
Reprod Sci Autophagy Contributes to Oxidative Stress-Induced Apoptosis in Porcine Granulosa Cells. | ||
J Cell Mol Med DEPDC1 up-regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells. |