pan Ras Monoclonal antibody

• Featured Product
• KD/KO Validated

pan Ras Monoclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IHC, FC,ELISA

Conjugate

Unconjugated

Publications

8

CloneNo.

4H4G7

Cat no : 60309-1-Ig

Synonyms

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2B speicifc, Krasa, Krasb, NS3, pan Ras, RASK2

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Validation Data Gallery

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  WB analysis using 60309-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 60309-1-Ig

  IP result of anti-pan Ras (IP:60309-1-Ig, 5ug; Detection:60309-1-Ig 1:2000) with mouse brain tissue lysate 4000 ug.

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  IHC staining of human breast cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60309-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 60309-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug pan Ras antibody (60309-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: fetal human brain tissue, HEK-293 cells, Neuro-2a cells, ROS1728 cells, human brain tissue, rat brain tissue, mouse brain tissue, pig brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below

Product Information

60309-1-Ig targets pan Ras in WB, IP, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: pan Ras fusion protein Ag2700
• Full Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Calculated molecular weight: 188 aa, 21 kDa
• Observed molecular weight: 21 kDa
• GenBank accession number: BC013572
• Gene symbol: KRAS
• Gene ID (NCBI): 3845
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.

Publications

Species	Application	Title
human	WB	Biomed Pharmacother; Modulation of YrdC promotes hepatocellular carcinoma progression via MEK/ERK signaling pathway.; Authors - Shiqiong Huang
human	WB,IHC	Heliyon; FOXP1 forkhead transcription factor is associated with the pathogenesis of endometrial cancer.; Authors - Makito Mizunuma
human	WB	Oncol Rep; EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells.; Authors - Chuan Lin
human	IHC	Int J Mol Med; Overexpression of wild-type p21Ras plays a prominent role in colorectal cancer.; Authors - Shuang Bai
pig	WB	Reprod Sci; Autophagy Contributes to Oxidative Stress-Induced Apoptosis in Porcine Granulosa Cells.; Authors - Jia-Qing Zhang
human	WB	J Cell Mol Med; DEPDC1 up-regulates RAS expression to inhibit autophagy in lung adenocarcinoma cells.; Authors - Wei Wang