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CoraLite®594-conjugated pan Ras Monoclonal antibody

pan Ras Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4H4G7

Cat no : CL594-60309

Synonyms

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2A, KRAS2B, KRAS2B speicifc, Krasa, Krasb, NS3, pan Ras, RASK2



Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60309-1-Ig

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL594-60309 targets pan Ras in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen pan Ras fusion protein Ag2700
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 188 aa, 21 kDa
GenBank accession numberBC013572
Gene symbol KRAS
Gene ID (NCBI) 3845
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm / 614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.

Protocols

Product Specific Protocols
FC protocol for CL594 pan Ras antibody CL594-60309Download protocol
Standard Protocols
Click here to view our Standard Protocols