CoraLite®555-conjugated pan Ras Monoclonal antibody

• Featured Product
• KD/KO Validated

pan Ras Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4H4G7

Cat no : CL555-60309

Synonyms

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2A, KRAS2B, KRAS2B speicifc, Krasa, Krasb, NS3, RASK2

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Validation Data Gallery

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  FC experiment of HeLa using CL555-60309

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human pan Ras (CL555-60309, Clone:4H4G7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-60309 targets pan Ras in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: pan Ras fusion protein Ag2700
• Full Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Calculated Molecular Weight: 188 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC013572
• Gene Symbol: KRAS
• Gene ID (NCBI): 3845
• RRID: AB_2919662
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C