Cytokeratin 1-specific Polyclonal antibody

Cytokeratin 1-specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 16848-1-AP

Synonyms

Cytokeratin 1, CK-1, K1, Cytokeratin-1, Cytokeratin1

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Validation Data Gallery

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  WB analysis using 16848-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skin using 16848-1-AP

  IP result of anti-Cytokeratin 1-specific (IP:16848-1-AP, 4ug; Detection:16848-1-AP 1:800) with mouse skin tissue lysate 640 ug.

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  IHC staining of human skin using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of rat skin using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skin using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse skin using 16848-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 1-specific antibody (16848-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 16848-1-AP

  Immunofluorescent analysis of A431 cells using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 16848-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 1-specific antibody (16848-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: rat skin tissue, mouse skin
• Positive IP detected in: mouse skin tissue
• Positive IHC detected in: rat skin tissue, human lung cancer tissue, human skin tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skin tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 7 publications below

Product Information

16848-1-AP targets Cytokeratin 1-specific in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: NM_006121
• Gene Symbol: KRT1
• Gene ID (NCBI): 3848
• RRID: AB_2134417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. This antibody is specifically against ketatin I.

Publications

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB,IF	J Invest Dermatol; Nuclear IL-33 plays an important role in the suppression of filaggrin, loricrin, keratin 1, and keratin 10 by IL-4 and IL-13 in human keratinocytes.; Authors - Xiuju Dai
human	WB	J Invest Dermatol; IL-22 downregulates peptidylarginine deiminase-1 in human keratinocytes: adding another piece to the IL-22 puzzle in epidermal barrier formation.; Authors - Avinash Padhi
mouse	WB,IF	Int J Mol Sci; Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm.; Authors - Xiaoning Zhang
human	IF	Sci Rep; Loss-of-function Mutation in PMVK Causes Autosomal Dominant Disseminated Superficial Porokeratosis.; Authors - Jiuxiang Wang
human	WB	Front Endocrinol (Lausanne); c-Myc Upregulated by High Glucose Inhibits HaCaT Differentiation by S100A6 Transcriptional Activation.; Authors - Jie Zhang

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