Cytokeratin 1-specific Polyclonal antibody

Cytokeratin 1-specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 16848-1-AP

Synonyms

67 kDa cytokeratin, CK 1, CK1, Cytokeratin 1, keratin 1, KRT1

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Validation Data Gallery

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  WB analysis using 16848-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 16848-1-AP

  IP Result of anti-Cytokeratin 1-specific (IP:16848-1-AP, 3ug; Detection:16848-1-AP 1:500) with A431 cells lysate 3500ug.

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  IHC staining of human skin using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 16848-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 16848-1-AP

  Immunofluorescent analysis of A431 cells using 16848-1-AP (Cytokeratin 1-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: rat skin tissue, mouse skin
• Positive IP detected in: A431 cells
• Positive IHC detected in: human skin tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

16848-1-AP targets Cytokeratin 1-specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 1
• Calculated molecular weight: 66 kDa
• Observed molecular weight: 67 kDa
• GenBank accession number: NM_006121
• Gene symbol: KRT1
• Gene ID (NCBI): 3848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. This antibody is specifically against ketatin I.

Publications

Species	Application	Title
mouse	WB,IF	Int J Mol Sci; Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm.; Authors - Xiaoning Zhang
human	WB	Exp Ther Med; Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.; Authors - Min Li
human	WB	Iran J Basic Med Sci; Inactivation of mitogen-activated protein kinase signaling pathway reduces caspase-14 expression in impaired keratinocytes.; Authors - Ningning Dang
goat	WB	Animals (Basel); Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications.; Authors - Sen Ma
mouse	IHC	Life Sci; The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model.; Authors - Shidi Wu
human	IHC,IF	Hum Cell; Histone deacetylase 6-mediated downregulation of TMEM100 expedites the development and progression of non-small cell lung cancer.; Authors - Yanyun Wang

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