Cytokeratin 1-specific Polyclonal antibody
Cytokeratin 1-specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, IP, IHC, IF, ELISA
Cat no : 16848-1-AP
Validation Data Gallery
|Positive WB detected in||rat skin tissue, mouse skin|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human skin tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|WB||See 7 publications below|
|IHC||See 2 publications below|
|IF||See 5 publications below|
16848-1-AP targets Cytokeratin 1-specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, goat|
|Host / Isotype||Rabbit / IgG|
|Full Name||keratin 1|
|Calculated molecular weight||66 kDa|
|Observed molecular weight||67 kDa|
|GenBank accession number||NM_006121|
|Gene ID (NCBI)||3848|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. This antibody is specifically against ketatin I.
|Product Specific Protocols|
|WB protocol for Cytokeratin 1-specific antibody 16848-1-AP||Download protocol|
|IHC protocol for Cytokeratin 1-specific antibody 16848-1-AP||Download protocol|
|IF protocol for Cytokeratin 1-specific antibody 16848-1-AP||Download protocol|
|IP protocol for Cytokeratin 1-specific antibody 16848-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Invest Dermatol
IL-22 downregulates peptidylarginine deiminase-1 in human keratinocytes: adding another piece to the IL-22 puzzle in epidermal barrier formation.
J Invest Dermatol
Nuclear IL-33 plays an important role in the suppression of filaggrin, loricrin, keratin 1, and keratin 10 by IL-4 and IL-13 in human keratinocytes.
Loss-of-function Mutation in PMVK Causes Autosomal Dominant Disseminated Superficial Porokeratosis.
Front Endocrinol (Lausanne)
c-Myc Upregulated by High Glucose Inhibits HaCaT Differentiation by S100A6 Transcriptional Activation.
Histone deacetylase 6-mediated downregulation of TMEM100 expedites the development and progression of non-small cell lung cancer.
The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model.