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Cytokeratin 1-specific Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 16848-1-AP

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Con: 52 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Cytokeratin 1-specific Antibody IHC human skin tissue 16848-1-AP

Immunohistochemical of paraffin-embedded human skin using 16848-1-AP(Cytokeratin 1-specific antibody) at dilution of 1:100 (under 10x lens)

Cytokeratin 1-specific Antibody IHC human skin tissue 16848-1-AP

Immunohistochemical of paraffin-embedded human skin using 16848-1-AP(Cytokeratin 1-specific antibody) at dilution of 1:100 (under 40x lens)

Cytokeratin 1-specific Antibody IP A431 cells 16848-1-AP

IP Result of anti-Cytokeratin 1-specific (IP:16848-1-AP, 3ug; Detection:16848-1-AP 1:500) with A431 cells lysate 3500ug.

Cytokeratin 1-specific Antibody WB mouse skin tissue 16848-1-AP

mouse skin tissue were subjected to SDS PAGE followed by western blot with 16848-1-AP( Cytokeratin 1-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

Cytokeratin 1-specific Antibody IF A431 cells 16848-1-AP

Immunofluorescent analysis of A431 cells using 16848-1-AP(Cytokeratin 1-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
mouse skin tissue, A375 cells, A431 cells
Positive IP detected in:
A431 cells
Positive IHC detected in:
human skin tissue, human cervix tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
A431 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
keratin 1
Calculated molecular weight:
66 kDa
Observed molecular weight:
67 kDa
GenBank accession number:
NM_006121
Gene ID (NCBI):
3848
Gene symbol
KRT1
Synonyms
67 kDa cytokeratin, CK 1, CK1, Cytokeratin 1, keratin 1, KRT1

Background

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. This antibody is specifically against ketatin I.


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