Cytokeratin 10-specific Polyclonal antibody

Cytokeratin 10-specific Polyclonal Antibody for FC, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 16855-1-AP

Synonyms

CK 10, CK10, Cytokeratin 10, K10, keratin 10, KPP, KRT10



Tested Applications

Positive IHC detected inhuman skin cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16855-1-AP targets Cytokeratin 10-specific in IHC, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name keratin 10
Calculated molecular weight 59 kDa
GenBank accession numberNM_000421
Gene symbol KRT10
Gene ID (NCBI) 3858
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia. This antibody is specifically against KRT10.

Protocols

Product Specific Protocols
IHC protocol for Cytokeratin 10-specific antibody 16855-1-APDownload protocol
FC protocol for Cytokeratin 10-specific antibody 16855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols