Cytokeratin 10-specific Polyclonal antibody
Cytokeratin 10-specific Polyclonal Antibody for FC, IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IHC, FC,ELISA
Conjugate
Unconjugated
Cat no : 16855-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human skin cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
16855-1-AP targets Cytokeratin 10-specific in IHC, FC,ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | keratin 10 |
Calculated molecular weight | 59 kDa |
GenBank accession number | NM_000421 |
Gene symbol | KRT10 |
Gene ID (NCBI) | 3858 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia. This antibody is specifically against KRT10.
Protocols
Product Specific Protocols | |
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IHC protocol for Cytokeratin 10-specific antibody 16855-1-AP | Download protocol |
FC protocol for Cytokeratin 10-specific antibody 16855-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |