Cytokeratin 10-specific Polyclonal antibody

Cytokeratin 10-specific Polyclonal Antibody for FC, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 16855-1-AP

Synonyms

CK 10, CK10, Cytokeratin 10, K10, keratin 10, KPP, KRT10

---

Validation Data Gallery

• 

  IHC staining of human skin cancer using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A431 using 16855-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human Cytokeratin 10-specific (16855-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

---

Tested Applications

• Positive IHC detected in: human skin cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

16855-1-AP targets Cytokeratin 10-specific in IHC, FC,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 10
• Calculated molecular weight: 59 kDa
• GenBank accession number: NM_000421
• Gene symbol: KRT10
• Gene ID (NCBI): 3858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia. This antibody is specifically against KRT10.