Cytokeratin 10-specific Polyclonal antibody

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  WB analysis using 16855-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skin using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skin using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HaCaT using 16855-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 10-specific antibody (16855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Cytokeratin 10-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16855-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KRT10, Cytokeratin-10, Cytokeratin,Keratin, Cytokeratin 10, CK-10

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Tested Applications

• Positive WB detected in: mouse skin tissue, rat skin tissue
• Positive IHC detected in: human skin cancer tissue, human breast cancer tissue, mouse skin tissue, rat skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HaCaT cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16855-1-AP targets Cytokeratin 10-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 10
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: NM_000421
• Gene Symbol: Cytokeratin 10
• Gene ID (NCBI): 3858
• RRID: AB_10792092
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13645
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia. This antibody is specifically against KRT10.