Cytokeratin 7 Polyclonal antibody

Cytokeratin 7 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15539-1-AP

Synonyms

Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7



Tested Applications

Positive WB detected inHeLa cells, rat liver tissue, A431 cells, mouse liver tissue, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman pancreas tissue, human breast cancer tissue, human kidney tissue, human liver cancer tissue, human lung tissue, human lung cancer tissue, human ovary tumor tissue, human stomach cancer tissue, mouse kidney tissue, mouse pancreas tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, mouse pancreas tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:30000
Immunofluorescence (IF)IF : 1:350-1:1400
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15539-1-AP targets Cytokeratin 7 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 7 fusion protein Ag7895
Full Name keratin 7
Calculated Molecular Weight 469 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC002700
Gene Symbol KRT7
Gene ID (NCBI) 3855
RRIDAB_2249769
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 7 antibody 15539-1-APDownload protocol
IHC protocol for Cytokeratin 7 antibody 15539-1-APDownload protocol
IF protocol for Cytokeratin 7 antibody 15539-1-APDownload protocol
FC protocol for Cytokeratin 7 antibody 15539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cell

Neurulation of the cynomolgus monkey embryo achieved from 3D blastocyst culture

Authors - Jinglei Zhai
mouseIHC

Cancer Cell

Promotion of cholangiocarcinoma growth by diverse cancer-associated fibroblast subpopulations.

Authors - Silvia Affo
humanIF

Cell Death Dis

α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation

Authors - Hao Wang
humanWB,IHC,IF

Apoptosis

PANoptosis subtypes predict prognosis and immune efficacy in gastric cancer

Authors - Zitao Liu
humanWB, IHC, IF

Cell Death Dis

LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface.

Authors - S Liu
ratIHC

J Ethnopharmacol

Astragaloside prevents BDL-induced liver fibrosis through inhibition of notch signaling activation.

Authors - Mu Yongping