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Cytokeratin 7 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 15539-1-AP

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

Cytokeratin 7 Antibody WB HepG2 cells 15539-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 15539-1-AP(KRT7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Cytokeratin 7 Antibody IF HeLa cells 15539-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 15539-1-AP(Cytokeratin 7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Cytokeratin 7 Antibody IHC human pancreas tissue 15539-1-AP

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:25600 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Cytokeratin 7 Antibody IHC human pancreas tissue 15539-1-AP

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:25600 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Cytokeratin 7 Antibody FC HeLa cells 15539-1-AP

1X10^6 HeLa cells were stained with 0.2ug Cytokeratin 7 antibody (15539-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
HepG2 cells, rat liver tissue, A431 cells, HeLa cells, mouse liver tissue
Positive IHC detected in:
human pancreas tissue, human liver cancer tissue, mouse lung tissue, rat ovary tissue, human stomach cancer tissue, rat stomach tissue, human lung cancer tissue, human breast cancer tissue, human ovary tumor tissue
Positive IF detected in:
HeLa cells, HepG2 cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:1000-1:30000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Cytokeratin 7 fusion protein Ag7895
Full name:
keratin 7
Calculated molecular weight:
469aa,51 kDa
Observed molecular weight:
51 kDa
GenBank accession number:
BC002700
Gene ID (NCBI):
3855
Gene symbol
KRT7
Synonyms
CK 7, CK7, Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

Background

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.


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