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Cytokeratin 7 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 15539-1-AP

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

Cytokeratin 7 Antibody WB HepG2 cells 15539-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 15539-1-AP(KRT7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Cytokeratin 7 Antibody IF HeLa cells 15539-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 15539-1-AP(Cytokeratin 7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Cytokeratin 7 Antibody IHC human pancreas tissue 15539-1-AP

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:25600 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Cytokeratin 7 Antibody IHC human pancreas tissue 15539-1-AP

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:25600 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Cytokeratin 7 Antibody FC HeLa cells 15539-1-AP

1X10^6 HeLa cells were stained with 0.2ug Cytokeratin 7 antibody (15539-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
HepG2 cells, rat liver tissue, A431 cells, HeLa cells, mouse liver tissue
Positive IHC detected in:
human pancreas tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, human ovary tumor tissue, human stomach cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:1000-1:30000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Cytokeratin 7 fusion protein Ag7895
Full name:
keratin 7
Calculated molecular weight:
469aa,51 kDa
Observed molecular weight:
51 kDa
GenBank accession number:
BC002700
Gene ID (NCBI):
3855
Gene symbol
KRT7
Synonyms
CK 7, CK7, Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

Background

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.


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