Cytokeratin 7 Polyclonal antibody
Cytokeratin 7 Polyclonal Antibody for FC, IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 15539-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, rat liver tissue, A431 cells, HeLa cells, mouse liver tissue |
| Positive IHC detected in | human pancreas tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, human ovary tumor tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:30000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
| IHC | See 5 publications below |
| IF | See 2 publications below |
Product Information
15539-1-AP targets Cytokeratin 7 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cytokeratin 7 fusion protein Ag7895 |
| Full Name | keratin 7 |
| Calculated molecular weight | 469 aa, 51 kDa |
| Observed molecular weight | 51 kDa |
| GenBank accession number | BC002700 |
| Gene symbol | KRT7 |
| Gene ID (NCBI) | 3855 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 7 antibody 15539-1-AP | Download protocol |
| IHC protocol for Cytokeratin 7 antibody 15539-1-AP | Download protocol |
| IF protocol for Cytokeratin 7 antibody 15539-1-AP | Download protocol |
| FC protocol for Cytokeratin 7 antibody 15539-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Astragaloside prevents BDL-induced liver fibrosis through inhibition of notch signaling activation. | ||
Reprod Biol Endocrinol Modulatory effects of trophoblast-secreted CXCL12 on the migration and invasion of human first-trimester decidual epithelial cells are mediated by CXCR4 rather than CXCR7. | ||
Am. J. Chin. Med. Huang Qi Decoction Prevents BDL-Induced Liver Fibrosis Through Inhibition of Notch Signaling Activation. | ||
Stem Cell Res Ther Establishment of human trophoblast stem cells from human induced pluripotent stem cell-derived cystic cells under micromesh culture. | ||
Cell Death Dis LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface. |
