Validation Data Gallery
|Positive WB detected in||HeLa cells, rat liver tissue, A431 cells, mouse liver tissue, HepG2 cells, MCF-7 cells|
|Positive IHC detected in||human pancreas tissue, human stomach cancer tissue, human liver cancer tissue, human breast cancer tissue, human ovary tumor tissue, human lung tissue, human lung cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:30000|
|Immunofluorescence (IF)||IF : 1:350-1:1400|
|Sample-dependent, check data in validation data gallery|
15539-1-AP targets Cytokeratin 7 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytokeratin 7 fusion protein Ag7895|
|Full Name||keratin 7|
|Calculated molecular weight||469 aa, 51 kDa|
|Observed molecular weight||51 kDa|
|GenBank accession number||BC002700|
|Gene ID (NCBI)||3855|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.
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