Cytokeratin 7 Polyclonal antibody

Cytokeratin 7 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 15539-1-AP

Synonyms

Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

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Validation Data Gallery

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  WB analysis using 15539-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15539-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15539-1-AP (KRT7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 15539-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15539-1-AP (KRT7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15539-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15539-1-AP (KRT7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15539-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15539-1-AP (KRT7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:25600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:25600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15539-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 15539-1-AP

  1X10^6 HeLa cells were stained with 0.2 ug Anti-Human Cytokeratin 7 (15539-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

• 

  FC experiment of HeLa using 15539-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Cytokeratin 7 antibody (15539-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HeLa cells, rat liver tissue, A431 cells, mouse liver tissue, HepG2 cells, MCF-7 cells
• Positive IHC detected in: human pancreas tissue, human stomach cancer tissue, human liver cancer tissue, human breast cancer tissue, human ovary tumor tissue, human lung tissue, human lung cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:30000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IHC: See 7 publications below
• IF: See 4 publications below

Product Information

15539-1-AP targets Cytokeratin 7 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 7 fusion protein Ag7895
• Full Name: keratin 7
• Calculated molecular weight: 469 aa, 51 kDa
• Observed molecular weight: 51 kDa
• GenBank accession number: BC002700
• Gene symbol: KRT7
• Gene ID (NCBI): 3855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

Publications

Species	Application	Title
rat	IHC	J Ethnopharmacol; Astragaloside prevents BDL-induced liver fibrosis through inhibition of notch signaling activation.; Authors - Mu Yongping
rat	IHC	J Biomed Res; PMID: 30008464; Authors - Mu-Yi Yang
mouse	IHC	mBio; A Novel Model for Papillomavirus-Mediated Anal Disease and Cancer Using the Mouse Papillomavirus.; Authors - Simon Blaine-Sauer
human	IHC	Reprod Biol Endocrinol; Modulatory effects of trophoblast-secreted CXCL12 on the migration and invasion of human first-trimester decidual epithelial cells are mediated by CXCR4 rather than CXCR7.; Authors - Jiayi Zheng
human	IHC	Am. J. Chin. Med.; Huang Qi Decoction Prevents BDL-Induced Liver Fibrosis Through Inhibition of Notch Signaling Activation.; Authors - Xiao Zhang
human	IF	Stem Cell Res Ther; Establishment of human trophoblast stem cells from human induced pluripotent stem cell-derived cystic cells under micromesh culture.; Authors - Zhuosi Li

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