|Positive WB detected in||HepG2 cells, rat liver tissue, A431 cells, HeLa cells, mouse liver tissue|
|Positive IHC detected in||human pancreas tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, human ovary tumor tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:30000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15539-1-AP targets Cytokeratin 7 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytokeratin 7 fusion protein Ag7895|
|Full Name||keratin 7|
|Calculated molecular weight||469 aa, 51 kDa|
|Observed molecular weight||51 kDa|
|GenBank accession number||BC002700|
|Gene ID (NCBI)||3855|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.
|Product Specific Protocols|
|WB protocol for Cytokeratin 7 antibody 15539-1-AP||Download protocol|
|IHC protocol for Cytokeratin 7 antibody 15539-1-AP||Download protocol|
|IF protocol for Cytokeratin 7 antibody 15539-1-AP||Download protocol|
|FC protocol for Cytokeratin 7 antibody 15539-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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