Cytokeratin 7 Polyclonal antibody

Cytokeratin 7 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15539-1-AP

Synonyms

CK 7, CK7, Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7



Tested Applications

Positive WB detected inHepG2 cells, rat liver tissue, A431 cells, HeLa cells, mouse liver tissue
Positive IHC detected inhuman pancreas tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, human ovary tumor tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:30000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15539-1-AP targets Cytokeratin 7 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 7 fusion protein Ag7895
Full Name keratin 7
Calculated molecular weight 469 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC002700
Gene symbol KRT7
Gene ID (NCBI) 3855
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 7 antibody 15539-1-APDownload protocol
IHC protocol for Cytokeratin 7 antibody 15539-1-APDownload protocol
IF protocol for Cytokeratin 7 antibody 15539-1-APDownload protocol
FC protocol for Cytokeratin 7 antibody 15539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

J Ethnopharmacol

Astragaloside prevents BDL-induced liver fibrosis through inhibition of notch signaling activation.

Authors - Mu Yongping
ratIHC

J Biomed Res

PMID: 30008464

Authors - Mu-Yi Yang
humanIHC

Reprod Biol Endocrinol

Modulatory effects of trophoblast-secreted CXCL12 on the migration and invasion of human first-trimester decidual epithelial cells are mediated by CXCR4 rather than CXCR7.

Authors - Jiayi Zheng
humanIHC

Am. J. Chin. Med.

Huang Qi Decoction Prevents BDL-Induced Liver Fibrosis Through Inhibition of Notch Signaling Activation.

Authors - Xiao Zhang
humanIF

Stem Cell Res Ther

Establishment of human trophoblast stem cells from human induced pluripotent stem cell-derived cystic cells under micromesh culture.

Authors - Zhuosi Li
humanWB, IHC, IF

Cell Death Dis

LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface.

Authors - S Liu