LCMT2 Recombinant antibody

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  IF Staining of HepG2 using 83073-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCMT2 antibody (83073-2-RR, Clone: 230405H8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 83073-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug LCMT2 Recombinant antibody (83073-2-RR, Clone:230405H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

LCMT2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 83073-2-RR

Clone No.230405H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

tRNA yW-synthesizing protein 4, tRNA wybutosine-synthesizing protein 4, PPM2, KIAA0547, EC:2.1.1.290

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83073-2-RR targets LCMT2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LCMT2 fusion protein Ag11145
• Full Name: leucine carboxyl methyltransferase 2
• Calculated Molecular Weight: aa, 76 kDa
• GenBank Accession Number: BC015949
• Gene Symbol: LCMT2
• Gene ID (NCBI): 9836
• RRID: AB_3670792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O60294
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LCMT2, also known as tRNA wybutosine-synthesizing protein 4 (TYW4), is an S-adenosylmethionine (SAM)-dependent enzyme that catalyzes the final step of yW biosynthesis, methylation and methoxycarbonylation.LCMT2 has a conserved leucine carboxyl methyltransferase (LCM) domain in its N-terminal region (PMID: 16642040).