LCMT2 Recombinant monoclonal antibody, PBS Only

• 

  IF Staining of HepG2 using 83073-2-RR (same clone as 83073-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCMT2 antibody (83073-2-RR, Clone: 230405H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83073-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83073-2-RR (same clone as 83073-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug LCMT2 Recombinant antibody (83073-2-RR, Clone:230405H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83073-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83073-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83073-2-PBS against Human LCMT2 was performed. The affinity constant is 68.8 nM.

LCMT2 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83073-2-PBS

Clone No.230405H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

230405H8, EC:2.1.1.290, KIAA0547, PPM2, tRNA wybutosine-synthesizing protein 4

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Product Information

83073-2-PBS targets LCMT2 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11145

  Product name: Recombinant human LCMT2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 340-686 aa of BC015949

  Sequence: VFPASVVSSEGQVPNLKRYGHASVFLSPDVILSAGGFGEQEGRHCRVSQFHLLSRDCDSEWKGSQIGSCGTGVQWDGRLYHTMTRLSESRVLVLGGRLSPVSPALGVLQLHFFKSEDNNTEDLKVTITKAGRKDDSTLCCWRHSTTEVSCQNQEYLFVYGGRSVVEPVLSDWHFLHVGTMAWVRIPVEGEVPEARHSHSACTWQGGALIAGGLGASEEPLNSVLFLRPISCGFLWESVDIQPPITPRYSHTAHVLNGKLLLVGGIWIHSSSFPGVTVINLTTGLSSEYQIDTTYVPWPLMLHNHTSILLPEEQQLLLLGGGGNCFSFGTYFNPHTVTLDLSSLSAGQ

• Full Name: leucine carboxyl methyltransferase 2
• Calculated Molecular Weight: aa, 76 kDa
• GenBank Accession Number: BC015949
• Gene Symbol: LCMT2
• Gene ID (NCBI): 9836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60294
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.