LCMT2 Recombinant antibody, PBS Only (Detector)

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  IF Staining of HepG2 using 83073-2-RR (same clone as 83073-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCMT2 antibody (83073-2-RR, Clone: 230405H8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83073-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83073-2-RR (same clone as 83073-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug LCMT2 Recombinant antibody (83073-2-RR, Clone:230405H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83073-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00060-1

  Cytometric bead array standard curve ofMP00060-1, LCMT2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83073-1-PBS. Detection antibody: 83073-2-PBS. Standard: Ag11145. Range: 0.625-20 ng/mL.

LCMT2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83073-2-PBS

Clone No.230405H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

tRNA yW-synthesizing protein 4, tRNA wybutosine-synthesizing protein 4, PPM2, KIAA0547, EC:2.1.1.290

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Product Information

83073-2-PBS targets LCMT2 as part of a matched antibody pair:

MP00060-1: 83073-1-PBS capture and 83073-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LCMT2 fusion protein Ag11145
• Full Name: leucine carboxyl methyltransferase 2
• Calculated Molecular Weight: aa, 76 kDa
• GenBank Accession Number: BC015949
• Gene Symbol: LCMT2
• Gene ID (NCBI): 9836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60294
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.