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LDHA Monoclonal antibody

LDHA Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human, mouse, pig, rat







Cat no : 66287-1-Ig


lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, PIG19

Tested Applications

Positive WB detected inpig liver tissue, pig skeletal muscle tissue, A431 cells, rat skeletal muscle, mouse skeletal muscle
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66287-1-Ig targets LDHA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen LDHA fusion protein Ag21417
Full Name lactate dehydrogenase A
Calculated Molecular Weight 332 aa, 37 kDa
Observed Molecular Weight 32-37 kDa
GenBank Accession NumberBC067223
Gene Symbol LDHA
Gene ID (NCBI) 3939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies. Mutations in LDHA have been linked to exertional myoglobinuria. LDHA has some isoforms with MW 26-40 kDa.


Product Specific Protocols
WB protocol for LDHA antibody 66287-1-IgDownload protocol
IHC protocol for LDHA antibody 66287-1-IgDownload protocol
IF protocol for LDHA antibody 66287-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Metab

Augmentation of scleral glycolysis promotes myopia through histone lactylation

Authors - Xiaolei Lin

Life Sci

Aerobic exercise reduced the amount of CHRONO bound to BMAL1 and ameliorated glucose metabolic dysfunction in skeletal muscle of high-fat diet-fed mice

Authors - Lei Xu

J Cell Mol Med

PMID: 31945804

Authors - Chenyang Chen

Int J Biol Sci

LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.

Authors - Yan Fang

Front Oncol

Lactate Dehydrogenase B Is Required for Pancreatic Cancer Cell Immortalization Through Activation of Telomerase Activity.

Authors - Ruiguan Wang

Front Oncol

Ursolic Acid Inhibits Breast Cancer Metastasis by Suppressing Glycolytic Metabolism via Activating SP1/Caveolin-1 Signaling.

Authors - Shengqi Wang