LDHA Monoclonal antibody

LDHA Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

CloneNo.

2E2G6

Cat no : 66287-1-Ig

Synonyms

lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, PIG19

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Validation Data Gallery

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  WB analysis using 66287-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66287-1-Ig (LDHA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 66287-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66287-1-Ig (LDHA Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66287-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66287-1-Ig (LDHA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66287-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66287-1-Ig (LDHA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66287-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66287-1-Ig (LDHA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66287-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66287-1-Ig(LDHA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig liver tissue, pig skeletal muscle tissue, A431 cells, rat skeletal muscle, mouse skeletal muscle
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

66287-1-Ig targets LDHA in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: LDHA fusion protein Ag21417
• Full Name: lactate dehydrogenase A
• Calculated molecular weight: 332 aa, 37 kDa
• Observed molecular weight: 32-37 kDa
• GenBank accession number: BC067223
• Gene symbol: LDHA
• Gene ID (NCBI): 3939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies. Mutations in LDHA have been linked to exertional myoglobinuria. LDHA has some isoforms with MW 26-40 kDa.

Publications

Species	Application	Title
mouse	IHC	Int J Biol Sci; LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.; Authors - Yan Fang
human,mouse	WB,IF	Front Oncol; Ursolic Acid Inhibits Breast Cancer Metastasis by Suppressing Glycolytic Metabolism via Activating SP1/Caveolin-1 Signaling.; Authors - Shengqi Wang
human	WB	Front Oncol; Lactate Dehydrogenase B Is Required for Pancreatic Cancer Cell Immortalization Through Activation of Telomerase Activity.; Authors - Ruiguan Wang
human	IHC	J Cell Mol Med; FOXM1 regulates glycolysis in nasopharyngeal carcinoma cells through PDK1.; Authors - Qing Yang
human	WB	Front Mol Biosci; Accumulated ROS Activates HIF-1α-Induced Glycolysis and Exerts a Protective Effect on Sensory Hair Cells Against Noise-Induced Damage.; Authors - Shuo Liang
human	WB	Hum Cell; MiR-323a-3p suppressed the glycolysis of osteosarcoma via targeting LDHA.; Authors - Hanwen Chen