|Positive WB detected in||pig liver tissue, pig skeletal muscle tissue, A431 cells, rat skeletal muscle, mouse skeletal muscle|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66287-1-Ig targets LDHA in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig samples.
|Tested Reactivity||human, mouse, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||LDHA fusion protein Ag21417|
|Full Name||lactate dehydrogenase A|
|Calculated molecular weight||332 aa, 37 kDa|
|Observed molecular weight||28 kDa, 40 kDa|
|GenBank accession number||BC067223|
|Gene ID (NCBI)||3939|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies. Mutations in LDHA have been linked to exertional myoglobinuria. LDHA has some isoforms with MW 26-40 kDa.
LDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma.
J Cell Mol Med
Epigenomic and transcriptional profiling identifies impaired glyoxylate detoxification in NAFLD as a risk factor for hyperoxaluria.
MiR-323a-3p suppressed the glycolysis of osteosarcoma via targeting LDHA.