LDHA Monoclonal antibody
LDHA Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
human, mouse, pig and More (1)
WB, IHC, IF, ELISA
Cat no : 66287-1-Ig
|Positive WB detected in||pig liver tissue, pig skeletal muscle tissue, A431 cells, rat skeletal muscle, mouse skeletal muscle|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 7 publications below|
|IHC||See 4 publications below|
|IF||See 1 publications below|
66287-1-Ig targets LDHA in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig samples.
|Tested Reactivity||human, mouse, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||LDHA fusion protein Ag21417|
|Full Name||lactate dehydrogenase A|
|Calculated molecular weight||332 aa, 37 kDa|
|Observed molecular weight||32-37 kDa|
|GenBank accession number||BC067223|
|Gene ID (NCBI)||3939|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies. Mutations in LDHA have been linked to exertional myoglobinuria. LDHA has some isoforms with MW 26-40 kDa.
|Product Specific Protocols|
|WB protocol for LDHA antibody 66287-1-Ig||Download protocol|
|IHC protocol for LDHA antibody 66287-1-Ig||Download protocol|
|IF protocol for LDHA antibody 66287-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Int J Biol Sci
LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.
Ursolic Acid Inhibits Breast Cancer Metastasis by Suppressing Glycolytic Metabolism via Activating SP1/Caveolin-1 Signaling.
Lactate Dehydrogenase B Is Required for Pancreatic Cancer Cell Immortalization Through Activation of Telomerase Activity.
J Cell Mol Med
FOXM1 regulates glycolysis in nasopharyngeal carcinoma cells through PDK1.
Front Mol Biosci
Accumulated ROS Activates HIF-1α-Induced Glycolysis and Exerts a Protective Effect on Sensory Hair Cells Against Noise-Induced Damage.
MiR-323a-3p suppressed the glycolysis of osteosarcoma via targeting LDHA.