LDHD Polyclonal antibody

LDHD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14398-1-AP

Synonyms

Probable D-lactate dehydrogenase, mitochondrial, Lactate dehydrogenase D, EC:1.1.2.4, DLD

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Validation Data Gallery

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  WB analysis using 14398-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14398-1-AP (LDHD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14398-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14398-1-AP (LDHD antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14398-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14398-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14398-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14398-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 14398-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14398-1-AP (LDHD antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human osteosarcoma using 14398-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14398-1-AP (LDHD antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14398-1-AP

  Immunofluorescent analysis of HepG2 cells, using LDHD antibody 14398-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, rat liver tissue
• Positive IHC detected in: human liver cancer tissue, human osteosarcoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14398-1-AP targets LDHD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LDHD fusion protein Ag5897
• Full Name: lactate dehydrogenase D
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 45-54 kDa
• GenBank Accession Number: BC047902
• Gene Symbol: LDHD
• Gene ID (NCBI): 197257
• RRID: AB_2249831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Two naturally occurring forms of lactate dehydrogenase with similar but unique substrate specificities have been isolated in lower organisms including invertebrates, fungi, and prokaryotes. These dehydrogenase enzymes are L-lactate dehydrogenase and D-lactate dehydrogenase (LDHD) that are specific to the L and D isomers of lactate, respectively (PMID: 12127981). In lactic acid bacteria, LDHD plays a key role in anaerobic energy metabolism (PMID: 497162). Despite the identification of D-lactate and other D-2-hydroxyacids in prokaryotes, and the obvious connections and similarities to vertebrate metabolic pathways, very few mammalian D-2-hydroxyacid dehydrogenases have been found. LDHD has 2 isoforms with the molecular weight of 52 and 54kDa, and can be detected as 45-54 kDa.

Publications

Species	Application	Title
human	IHC,WB,IF	BMC Cancer; Lactate dehydrogenase D serves as a novel biomarker for prognosis and immune infiltration in lung adenocarcinoma; Authors - Yu Zhang
human	IHC,IP	Signal Transduct Target Ther; CDK7-YAP-LDHD axis promotes D-lactate elimination and ferroptosis defense to support cancer stem cell-like properties; Authors - Mengzhu Lv