LGALS3BP Polyclonal antibody

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  WB analysis using 10281-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10281-1-AP

  WB result of LGALS3BP antibody (10281-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LGALS3BP transfected HepG2 cells.

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  WB analysis of fetal human brain using 10281-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10281-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 10281-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10281-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10281-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10281-1-AP

  IP result of anti-LGALS3BP (IP:10281-1-AP, 4ug; Detection:10281-1-AP 1:1000) with HepG2 cells lysate 1085 ug.

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  IP experiment of HEK-293 using 10281-1-AP

  IP result of anti-LGALS3BP (IP:10281-1-AP, 4ug; Detection:10281-1-AP 1:400) with HEK-293 cells lysate 1000ug.

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  IHC staining of human oesophagus cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10281-1-AP (LGALS3BP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10281-1-AP (LGALS3BP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10281-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LGALS3BP antibody (10281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

LGALS3BP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10281-1-AP

Publications(21)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

LG3BP, Lectin galactoside-binding soluble 3-binding protein, Galectin-3-binding protein, Galectin 3 binding protein, BTBD17B

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Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells, HEK-293 cells, fetal human brain tissue, HepG2 cells, A549 cells, human milk tissue, human plasma tissue
• Positive IP detected in: HepG2 cells, HEK-293 cells
• Positive IHC detected in: human oesophagus cancer tissue, human breast cancer tissue, human colon cancer, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 7 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Product Information

10281-1-AP targets LGALS3BP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LGALS3BP fusion protein Ag0294
• Full Name: lectin, galactoside-binding, soluble, 3 binding protein
• Calculated Molecular Weight: 585 aa, 65 kDa
• Observed Molecular Weight: 65-90 kDa
• GenBank Accession Number: BC002403
• Gene Symbol: LGALS3BP
• Gene ID (NCBI): 3959
• RRID: AB_2137066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q08380
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
human	IHC	Cancer Res; A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.; Authors - Silverman Ayaka M AM ; KD Validated
human	IF	Blood Adv; Increased Mac-2 binding protein glycan isomer in patients at risk for late nonrelapse mortality after HSCT.; Authors - Yu Akahoshi
mouse	WB	PLoS Pathog; Inducible LGALS3BP/90K activates antiviral innate immune responses by targeting TRAF6 and TRAF3 complex.; Authors - Gang Xu
human	WB	Thromb Haemost; Proteomic discovery of 21 proteins expressed in human plasma-derived but not platelet-derived microparticles.; Authors - Smalley David M DM
human	IHC	Cancer Sci; MZT2A promotes NSCLC viability and invasion by increasing Akt phosphorylation via the MOZART2 domain.; Authors - Huanxi Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (03-15-2022)

Beautiful antibody - produces a single band at the correct size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: LNCaP