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- KD/KO Validated
LGALS3BP Polyclonal antibody
LGALS3BP Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10281-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, HEK-293 cells, A549 cells, HeLa cells, COLO 320 cells |
| Positive IP detected in | HepG2 cells, HEK-293 cells |
| Positive IHC detected in | human oesophagus cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
10281-1-AP targets LGALS3BP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LGALS3BP fusion protein Ag0294 |
| Full Name | lectin, galactoside-binding, soluble, 3 binding protein |
| Calculated molecular weight | 585 aa, 65 kDa |
| Observed molecular weight | 65-90 kDa |
| GenBank accession number | BC002403 |
| Gene symbol | LGALS3BP |
| Gene ID (NCBI) | 3959 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LGALS3BP antibody 10281-1-AP | Download protocol |
| IHC protocol for LGALS3BP antibody 10281-1-AP | Download protocol |
| IF protocol for LGALS3BP antibody 10281-1-AP | Download protocol |
| IP protocol for LGALS3BP antibody 10281-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Blood Adv Increased Mac-2 binding protein glycan isomer in patients at risk for late nonrelapse mortality after HSCT. | ||
Mol Med Rep Composition and characteristics of distinct macrophage subpopulations in the mouse thymus. | ||
Haemophilia Galectin 3-binding protein is a potential contaminant of recombinantly produced factor IX. | ||
Cancer Biol Ther SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome. | ||
Cell Signal Increased LGALS3BP promotes proliferation and migration of oral squamous cell carcinoma via PI3K/AKT pathway.
| ||
PLoS One O-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation. |
