LGALS3BP Polyclonal antibody

• Featured Product
• KD/KO Validated

LGALS3BP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Publications

14

Reviews (1)

Cat no : 10281-1-AP

Synonyms

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K

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Validation Data Gallery

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  WB analysis of fetal human brain using 10281-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10281-1-AP

  WB result of LGALS3BP antibody (10281-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LGALS3BP transfected HepG2 cells.

• 

  WB analysis of COLO 320 using 10281-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10281-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10281-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10281-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10281-1-AP

  IP Result of anti-LGALS3BP (IP:10281-1-AP, 4ug; Detection:10281-1-AP 1:300) with HepG2 cells lysate 3480ug.

• 

  IP experiment of HEK-293 using 10281-1-AP

  IP Result of anti-LGALS3BP (IP:10281-1-AP, 4ug; Detection:10281-1-AP 1:400) with HEK-293 cells lysate 1000ug.

• 

  IHC staining of human oesophagus cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10281-1-AP (LGALS3BP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10281-1-AP (LGALS3BP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10281-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10281-1-AP (LGALS3BP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, COLO 320 cells, HEK-293 cells, HepG2 cells, HeLa cells, A549 cells
• Positive IP detected in: HepG2 cells, HEK-293 cells
• Positive IHC detected in: human oesophagus cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

10281-1-AP targets LGALS3BP in WB, IP, IHC, IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LGALS3BP fusion protein Ag0294
• Full Name: lectin, galactoside-binding, soluble, 3 binding protein
• Calculated molecular weight: 585 aa, 65 kDa
• Observed molecular weight: 65-90 kDa
• GenBank accession number: BC002403
• Gene symbol: LGALS3BP
• Gene ID (NCBI): 3959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).

Publications

Species	Application	Title
human	IF	Blood Adv; Increased Mac-2 binding protein glycan isomer in patients at risk for late nonrelapse mortality after HSCT.; Authors - Yu Akahoshi
mouse	IF	Mol Med Rep; Composition and characteristics of distinct macrophage subpopulations in the mouse thymus.; Authors - Lan-Tao Liu
human	WB	Haemophilia; Galectin 3-binding protein is a potential contaminant of recombinantly produced factor IX.; Authors - Blostein M M
human	IHC	Cancer Biol Ther; SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome.; Authors - Kashyap Manoj Kumar MK
human	WB,IHC	Cell Signal; Increased LGALS3BP promotes proliferation and migration of oral squamous cell carcinoma via PI3K/AKT pathway.; Authors - Xiaoxin Zhang ; KD Validated
human	WB	PLoS One; O-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation.; Authors - Isono Takahiro T

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (03-15-2022)

Beautiful antibody - produces a single band at the correct size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: LNCaP

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