LIAS Polyclonal antibody

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• LIAS Polyclonal antibody (11577-1-AP)

LIAS Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11577-1-AP

Publications(199)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

EC:2.8.1.8, HUSSY 01, HUSSY-01, LAS, Lip syn

• 

  WB analysis using 11577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 11577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 11577-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 11577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of L02 using 11577-1-AP

  IP result of anti-LIAS (IP:11577-1-AP, 4ug; Detection:11577-1-AP 1:4000) with L02 cells lysate 1000 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse liver using 11577-1-AP

  IP result of anti-LIAS (IP:11577-1-AP, 4ug; Detection:11577-1-AP 1:4000) with mouse liver tissue lysate 2320 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 11577-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11577-1-AP (LIAS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 11577-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11577-1-AP (LIAS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lymphoma using 11577-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11577-1-AP (LIAS antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: L02 cells, HepG2 cells, human brain tissue, Raji cells, K-562 cells, mouse brain tissue, mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue, L02 cells
• Positive IHC detected in: human liver cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11577-1-AP targets LIAS in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2162

  Product name: Recombinant human LIAS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-372 aa of BC023635

  Sequence: MSLRCGDAARTLGPRVFGRYFCSPVRPLSSLPDKKKELLQNGPDLQDFVSGDLADRSTWDEYKGNLKRQKGERLRLPPWLKTEIPMGKNYNKLKNTLRNLNLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDASEPYNTAKAIAEWGLDYVVLTSVDRDDMPDGGAEHIAKTVSYLKERNPKILVECLTPDFRGDLKAIEKVALSGLDVYAHNVETVPELQSKVRDPRVNFDQSLRVLKHAKKVQPDVISKTSIMLGLGENDEQVYATMKALREADVDCLTLGQYMQPTRRHLKVEEYITPEKFKYWEKVGNELGFHYTASGPLVRSSYKAGEFFLKNLVAKRKTKDL

• Full Name: lipoic acid synthetase
• Calculated Molecular Weight: 372 aa, 42 kDa
• Observed Molecular Weight: 34-42 kDa
• GenBank Accession Number: BC023635
• Gene Symbol: LIAS
• Gene ID (NCBI): 11019
• RRID: AB_2135972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43766
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIAS(lipoyl synthase, mitochondrial) is also named as LAS and belongs to the radical SAM superfamily and lipoyl synthase family. It produces alpha-lipoic acid, an antioxidant and an essential cofactor in alpha-ketoacid dehydrogenase complexes, which participate in glucose oxidation and ATP generation(PMID:22021711). The deduced 373-amino acid protein has a calculated molecular mass of about 42 kD. The N-terminal 26 amino acids encode a potential mitochondrial targeting presequence that, upon removal, would result in a deduced mature protein of 347 amino acids with a molecular mass of about 39 kD(PMID:11389890). Defects in LIAS are a cause of pyruvate dehydrogenase lipoic acid synthetase deficiency (PDHLD).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang
human	WB	Science; Copper induces cell death by targeting lipoylated TCA cycle proteins.; Authors - Peter Tsvetkov
mouse,human	WB,IHC	Adv Sci (Weinh); Enzalutamide Sensitizes Castration-Resistant Prostate Cancer to Copper-Mediated Cell Death; Authors - Xiang Gao
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
mouse	WB	Small; A Copper-Manganese Based Nanocomposite Induces Cuproptosis and Potentiates Anti-Tumor Immune Responses; Authors - Luoyingzi Xie
mouse	WB	Biomaterials; Magnesium hexacyanoferrate mitigates sepsis-associated encephalopathy through inhibiting microglial activation and neuronal cuproptosis; Authors - Yabing Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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James (Verified Customer) (10-01-2025)

Excellent antibody with perfect detection by chemiluminescence. Have used at 1:1000 and 1:2000 dilutions.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human In vitro Cancer Cells