LIAS Polyclonal antibody

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  WB analysis using 11577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11577-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11577-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11577-1-AP (LIAS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11577-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11577-1-AP (LIAS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11577-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11577-1-AP (LIAS antibody) at dilution of 1:100 (under 40x lens).

LIAS Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11577-1-AP

Publications(76)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

HUSSY 01, LAS, LIAS, Lip syn, LIP1, Lipoate synthase, Lipoic acid synthase, lipoic acid synthetase, Lipoyl synthase, mitochondrial, LS

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Tested Applications

• Positive WB detected in: L02 cells, HepG2 cells, human brain tissue, Raji cells, K-562 cells, mouse brain tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 71 publications below
• IHC: See 13 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

11577-1-AP targets LIAS in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2162

  Product name: Recombinant human LIAS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-372 aa of BC023635

  Sequence: MSLRCGDAARTLGPRVFGRYFCSPVRPLSSLPDKKKELLQNGPDLQDFVSGDLADRSTWDEYKGNLKRQKGERLRLPPWLKTEIPMGKNYNKLKNTLRNLNLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDASEPYNTAKAIAEWGLDYVVLTSVDRDDMPDGGAEHIAKTVSYLKERNPKILVECLTPDFRGDLKAIEKVALSGLDVYAHNVETVPELQSKVRDPRVNFDQSLRVLKHAKKVQPDVISKTSIMLGLGENDEQVYATMKALREADVDCLTLGQYMQPTRRHLKVEEYITPEKFKYWEKVGNELGFHYTASGPLVRSSYKAGEFFLKNLVAKRKTKDL

• Full Name: lipoic acid synthetase
• Calculated Molecular Weight: 372 aa, 42 kDa
• Observed Molecular Weight: 34-42 kDa
• GenBank Accession Number: BC023635
• Gene Symbol: LIAS
• Gene ID (NCBI): 11019
• RRID: AB_2135972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43766
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIAS(lipoyl synthase, mitochondrial) is also named as LAS and belongs to the radical SAM superfamily and lipoyl synthase family. It produces alpha-lipoic acid, an antioxidant and an essential cofactor in alpha-ketoacid dehydrogenase complexes, which participate in glucose oxidation and ATP generation(PMID:22021711). The deduced 373-amino acid protein has a calculated molecular mass of about 42 kD. The N-terminal 26 amino acids encode a potential mitochondrial targeting presequence that, upon removal, would result in a deduced mature protein of 347 amino acids with a molecular mass of about 39 kD(PMID:11389890). Defects in LIAS are a cause of pyruvate dehydrogenase lipoic acid synthetase deficiency (PDHLD).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang
human	WB	Science; Copper induces cell death by targeting lipoylated TCA cycle proteins.; Authors - Peter Tsvetkov
human,mouse	WB,IHC	Adv Sci (Weinh); Enzalutamide Sensitizes Castration-Resistant Prostate Cancer to Copper-Mediated Cell Death; Authors - Xiang Gao
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
mouse	WB	Small; A Copper-Manganese Based Nanocomposite Induces Cuproptosis and Potentiates Anti-Tumor Immune Responses; Authors - Luoyingzi Xie
mouse	WB	Biomaterials; Magnesium hexacyanoferrate mitigates sepsis-associated encephalopathy through inhibiting microglial activation and neuronal cuproptosis; Authors - Yabing Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (10-01-2025)

Excellent antibody with perfect detection by chemiluminescence. Have used at 1:1000 and 1:2000 dilutions.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human In vitro Cancer Cells