LIAS Polyclonal antibody

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  WB analysis using 11577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11577-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11577-1-AP (LIAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11577-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11577-1-AP (LIAS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11577-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11577-1-AP (LIAS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11577-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11577-1-AP (LIAS antibody) at dilution of 1:100 (under 40x lens).

LIAS Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11577-1-AP

Publications(56)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

HUSSY 01, LAS, LIAS, Lip syn, LIP1, Lipoate synthase, Lipoic acid synthase, lipoic acid synthetase, Lipoyl synthase, mitochondrial, LS

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Tested Applications

• Positive WB detected in: L02 cells, HepG2 cells, human brain tissue, Raji cells, K-562 cells, mouse brain tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 53 publications below
• IHC: See 9 publications below
• IF: See 3 publications below

Product Information

11577-1-AP targets LIAS in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIAS fusion protein Ag2162
• Full Name: lipoic acid synthetase
• Calculated Molecular Weight: 372 aa, 42 kDa
• Observed Molecular Weight: 34-42 kDa
• GenBank Accession Number: BC023635
• Gene Symbol: LIAS
• Gene ID (NCBI): 11019
• RRID: AB_2135972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43766
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIAS(lipoyl synthase, mitochondrial) is also named as LAS and belongs to the radical SAM superfamily and lipoyl synthase family. It produces alpha-lipoic acid, an antioxidant and an essential cofactor in alpha-ketoacid dehydrogenase complexes, which participate in glucose oxidation and ATP generation(PMID:22021711). The deduced 373-amino acid protein has a calculated molecular mass of about 42 kD. The N-terminal 26 amino acids encode a potential mitochondrial targeting presequence that, upon removal, would result in a deduced mature protein of 347 amino acids with a molecular mass of about 39 kD(PMID:11389890). Defects in LIAS are a cause of pyruvate dehydrogenase lipoic acid synthetase deficiency (PDHLD).

Publications

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang
human	WB	Science; Copper induces cell death by targeting lipoylated TCA cycle proteins.; Authors - Peter Tsvetkov
human,mouse	WB,IHC	Adv Sci (Weinh); Enzalutamide Sensitizes Castration-Resistant Prostate Cancer to Copper-Mediated Cell Death; Authors - Xiang Gao
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
mouse	WB,IF	Adv Healthc Mater; Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation; Authors - Ning Wang
mouse	WB	Acta Biomater; A metal-organic framework functionalized CaO2-based cascade nanoreactor induces synergistic cuproptosis/ferroptosis and Ca2+ overload-mediated mitochondrial damage for enhanced sono-chemodynamic immunotherapy; Authors - Cong Tang