LMOD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

LMOD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (1)

Cat no : 14948-1-AP

Synonyms

Leiomodin 3, leiomodin 3 (fetal), Leiomodin, fetal form, LMOD3

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Validation Data Gallery

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  WB analysis using 14948-1-AP

  Mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 14948-1-AP (LMOD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 14948-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14948-1-AP (LMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14948-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14948-1-AP (LMOD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 14948-1-AP

  IP result of anti-LMOD3 (IP:14948-1-AP, 4ug; Detection:14948-1-AP 1:700) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human heart using 14948-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14948-1-AP (LMOD3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 14948-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14948-1-AP (LMOD3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 14948-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14948-1-AP (LMOD3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 14948-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14948-1-AP (LMOD3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Product Information

14948-1-AP targets LMOD3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish, xenopus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LMOD3 fusion protein Ag6758
• Full Name: leiomodin 3 (fetal)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC039202
• Gene Symbol: LMOD3
• Gene ID (NCBI): 56203
• RRID: AB_2136690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gene encoding LMOD3 has not been characterized so far and very limited information of its function has been reported. Nanda et al. found that the expression of mouse LMOD3 mRNA is restricted largely to cardiac and skeletal muscle through RT-PCR analysis (PMID: 22157009). Two isoforms of LMOD3 may exist due to the alternative splicing, whose molecular weights are predicted as 65 kDa and 40 kDa, respectively (Uniprot). This antibody was raised against the N-terminal region of human LMOD3. It detects a double bands around 80 kDa and 65 kDa in heart and skeletal muscle lysates. The reason causing the discrepancy between the predicted and observed molecular weight is not clear.

Publications

Species	Application	Title
mouse	WB	Sci Adv; Nebulin and Lmod2 are critical for specifying thin-filament length in skeletal muscle.; Authors - Balázs Kiss
human, zebrafish	WB, IF	J Clin Invest; Leiomodin-3 dysfunction results in thin filament disorganization and nemaline myopathy.; Authors - Michaela Yuen
mouse	WB	J Clin Invest; Severe myopathy in mice lacking the MEF2/SRF-dependent gene leiomodin-3.; Authors - Bercin K Cenik ; KO Validated
zebrafish	WB, IF	J Clin Invest; Leiomodin-3 dysfunction results in thin filament disorganization and nemaline myopathy.; Authors - Michaela Yuen
human	WB	J Clin Invest; KLHL40 deficiency destabilizes thin filament proteins and promotes nemaline myopathy.; Authors - Ankit Garg
mouse	WB	Proc Natl Acad Sci U S A; HSPB7 is indispensable for heart development by modulating actin filament assembly.; Authors - Tongbin Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody is very specific. I had a single Lmod3 band at around 70kD. Western blot results of this antibody had been published in our PNAS paper (PMID: 29078393)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart

LMOD3 Antibody Western Blot validation (1:1000 dilution) in Heart (Cat no:14948-1-AP)